Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97

被引：91

作者：

Gálvez, A

Valdivia, E

Abriouel, H

Camafeita, E

Mendez, E

Martínez-Bueno, M

Maqueda, M

机构：

[1] Univ Jaen, Fac Ciencias Expt, Dept Microbiol, E-23071 Jaen, Spain

[2] Univ Granada, Fac Ciencias, Dept Microbiol, E-18071 Granada, Spain

[3] CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

[4] Univ Granada, Inst Biotecnol, E-18071 Granada, Spain

来源：

ARCHIVES OF MICROBIOLOGY | 1998年 / 171卷 / 01期

关键词：

bacteriocin; Enterococcus; Listeria; cationic peptide;

D O I：

10.1007/s002030050678

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The bacteriocinogenic strain of Enterococcus faecalis ET97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N-terminal residues of enterocin EJ97 indicates that it is different from other known protein sequences. Enterocin EJ97 is active on several gram-positive bacteria including enterococci, several species of Bacillus, Listeria, and Staphylococcus aureus. The producer strain is immune to bacteriocin. Enterocin EJ97 has a concentration-dependent bactericidal and bacteriolytic effect on E. faecalis S-47.

引用

页码：59 / 65

页数：7

共 42 条

[1] Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins [J].