Fluorescence spectroscopy determination of fluoroquinolones by charge-transfer reaction

The charge-transfer (CT) reaction between chloranilic acid (CL) as a pi-electron acceptor and lomefloxacin (LOM), fleroxacin (FLX), ciprofloxacin (CPFX), norfloxacin (NOR) as electron donor have been studied by fluorimetry. The CT complexes have stable purple color in acetone solution and the fluorescence intensity of the CT complexes was enhanced in 4-14 fold higher than that of the four fluoroquinolones (FQS) itself, therefore a new spectrofluorimetric method with simple, rapid, accurate, high sensitivity and good selectivity for determination of the four FQS has been developed. The method was applied for determination of drugs (LOM, FLX, CPFX and NOR) in tablets with mean percentage accuracies 99.80+/-1.12, 99.93+/-0.92, 99.23+/-1.36 and 99.87+/-0.81, respectively. (C) 2003 Elsevier B.V. All rights reserved.