Multiplexed and simultaneous biosensing in a 3D-printed portable six-well smartphone operated electrochemiluminescence standalone point-of-care platform

被引:23
作者
Bhaiyya, Manish [1 ]
Pattnaik, Prasant Kumar [1 ]
Goel, Sanket [1 ]
机构
[1] Birla Inst Technol & Sci Pilani, Dept Elect & Elect Engn, MEMS, Microfluid & Nanoelect Lab, Hyderabad Campus, Hyderabad 500078, India
关键词
3D printing; Electrochemiluminescence; Closed bipolar device; Biochemical sensing; Point-of-care testing; PAPER DEVICE; BIPOLAR; GENERATION; SYSTEM;
D O I
10.1007/s00604-022-05200-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
3D-printed portable devices have immense and proven potential to transform the field of electrochemiluminescence (ECL) for diverse biochemical applications. 3D printing (3DP) offers unparalleled ability to build tiny devices in a single step with high accuracy and compatibility, and integrability as per the requirement. In this study, for the first time, a six-well 3D-printed closed bipolar electrochemiluminescence (3DP-CBPE-ECL) device has been successfully fabricated and validated by performing single-step detection of various biochemicals such as glucose and choline. Luminol/H2O2-based enzymatic reactions were performed with optimized parameters for selective sensing of glucose and choline. The single-step detection of glucose and choline was accomplished for the linear ranges of 0.1 to 10 mM and 0.1 to 5 mM, with a limit of detections (LODs) of 24 mu M and 10 mu M, respectively. A smartphone was leveraged to execute multiple activities such as powering the ECL device, capturing ECL images, and calculating the ECL intensity of the obtained ECL signal. The feasibility of a six-well 3DP-CBPE-ECL device was tested by sensing glucose and choline simultaneously in a single device at three different concentrations. Furthermore, the concentration of glucose and choline was calculated in real blood serum using the conventional additive (spiking) method, demonstrating the high practicability of the fabricated ECL device and yielding promising findings. Finally, based on the obtained results and other advantages such as low-cost, fast prototyping and requirement of a minimum sample volume, the fabricated six-well 3DP-CBPE-ECL device has shown potential to be used in the field of biochemical applications.
引用
收藏
页数:9
相关论文
共 36 条
[21]   Open bipolar electrode-electrochemiluminescence imaging sensing using paper-based microfluidics [J].
Liu, Rui ;
Zhang, Chunsun ;
Liu, Min .
SENSORS AND ACTUATORS B-CHEMICAL, 2015, 216 :255-262
[22]   Conducting elastomer surface texturing: a path to electrode spotting - Application to the biochip production [J].
Marquette, CA ;
Blum, LJ .
BIOSENSORS & BIOELECTRONICS, 2004, 20 (02) :197-203
[23]   Optimized ink jetted paper device for electroanalytical detection of picric acid [J].
Mohan, Jaligam Murali ;
Amreen, Khairunnisa ;
Kulkarni, Madhusudan B. ;
Javed, Arshad ;
Dubey, Satish Kumar ;
Goel, Sanket .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2021, 208
[24]   Highly Sensitive and Interference-Free Electrochemical Nitrite Detection in a 3D Printed Miniaturized Device [J].
Pal, Abhishesh ;
Amreen, Khairunnisa ;
Dubey, Satish Kumar ;
Goel, Sanket .
IEEE TRANSACTIONS ON NANOBIOSCIENCE, 2021, 20 (02) :175-182
[25]   3D-Printed Graphene/Polylactic Acid Electrodes Promise High Sensitivity in Electroanalysis [J].
Palenzuela, C. Lorena Manzanares ;
Novotny, Filip ;
Krupicka, Petr ;
Sofer, Zdenek ;
Pumera, Martin .
ANALYTICAL CHEMISTRY, 2018, 90 (09) :5753-5757
[26]   Colorimetric and visual determination of hydrogen peroxide and glucose by applying paper-based closed bipolar electrochemistry [J].
Rafatmah, Elmira ;
Hemmateenejad, Bahram .
MICROCHIMICA ACTA, 2019, 186 (11)
[27]   Next-Generation 3D Printed Microfluidic Membraneless Enzymatic Biofuel Cell: Cost-Effective and Rapid Approach [J].
Rewatkar, Prakash ;
Goel, Sanket .
IEEE TRANSACTIONS ON ELECTRON DEVICES, 2019, 66 (08) :3628-3635
[28]  
Richter MM., 2004, ELECTROCHEMILUMINESC, DOI [10.1016/B978-044453125-4.50009-7, DOI 10.1016/B978-044453125-4.50009-7]
[29]   Development of Laser-Induced Graphene-Based Automated Electro Microfluidic Viscometer for Biochemical Sensing Applications [J].
Wagh, Mrunali D. ;
Puneeth, S. B. ;
Goel, Sanket ;
Sahoo, Subhendu Kumar .
IEEE TRANSACTIONS ON ELECTRON DEVICES, 2021, 68 (10) :5184-5191
[30]   A Simple and Sensitive Paper-Based Bipolar Electrochemiluminescence Biosensor for Detection of Oxidase-Substrate Biomarkers in Serum [J].
Wang, Dan ;
Liu, Cuiling ;
Liang, Yi ;
Su, Yan ;
Shang, Qiuping ;
Zhang, Chunsun .
JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2018, 165 (09) :B361-B369