1H, 13C and 15N chemical shift assignments for the human Pitx2 homeodomain and a R24H homeodomain mutant

被引：4

作者：

Doerdelmann, Thomas ^{[1
]}

Kojetin, Douglas J. ^{[1
]}

Baird-Titus, Jamie M. ^{[1
]}

Rance, Mark ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2011年 / 5卷 / 01期

关键词：

Pitx2; Homeodomain; Axenfeld-Rieger; DNA-binding; NMR; RIEGER-SYNDROME; MOUSE; GENE;

D O I：

10.1007/s12104-010-9278-x

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The homeodomain is one of the most important eukaryotic DNA-binding motifs and has been identified in over one thousand proteins. Homeodomain proteins play critical roles in diverse biological processes, including cell differentiation and cell pattern formation. The human Pitx2 homeodomain binds several different DNA sequences and is a pivotal component of both the TGF-beta and Wnt/beta-catenin signaling pathways. As the recognition of specific DNA sequences represents an essential biochemical function of all DNA-binding proteins, we have chosen the Pitx2 homeodomain model to investigate the mechanisms that convey biological specificity in these protein-DNA interactions. Here, we report complete chemical shift assignments of the human Pitx2 homeodomain and the R24H mutation that induces ring dermoid of the cornea syndrome.

引用

页码：105 / 107

页数：3

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