A droplet-based fluorescence polarization immunoassay (dFPIA) platform for rapid and quantitative analysis of biomarkers

被引：23

作者：

Choi, Jae-Won ^{[1
]}

Kim, Gil-Jung ^{[1
]}

Lee, Sangmin ^{[2
]}

Kim, Joonwon ^{[2
]}

deMello, Andrew J. ^{[3
]}

Chang, Soo-Ik ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Dept Biochem, Cheongju 361763, South Korea

[2] Pohang Univ Sci & Technol, Dept Mech Engn, Pohang 790784, South Korea

[3] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 67卷

基金：

新加坡国家研究基金会;

关键词：

Droplet-based microfluidics; Biomarker; Fluorescence polarization; Immunoassay; Angiogenin; Milk; PROTEIN-PROTEIN INTERACTIONS; MASS-SPECTROMETRY; BOVINE ANGIOGENIN; DISCOVERY; PEANUT; ASSAY;

D O I：

10.1016/j.bios.2014.09.013

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Herein, we describe for the first time the integration of pneumatic micro-pumps with droplet-based microfluidic systems as basic platform for the rapid detection and quantitation of biomarkers. Specifically, we combine this microfluidic platform with fluorescence polarization detection to identify and quantify the potent blood vessel inducing protein bovine angiogenin within cow's milk in high-throughput. The droplet-based fluorescence polarization immunoassay is successful in accurately determining the concentration (4.84 +/- 1.21 mu g/mL) of bovine angiogenin in cow's milk, affords a 10 fold reduction in dead volumes when compared to conventional droplet-based microfluidic experiments and requires an total sample volume of less than 1 nL. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：497 / 502

页数：6

共 44 条

[1] Biomarker discovery and applications for foods and beverages: Proteomics to nanoproteomics [J].