Diversity of Burkholderia cepacia Complex from the Moso Bamboo (Phyllostachys edulis) Rhizhosphere Soil

被引:6
作者
Lou, Miaomiao [1 ,2 ]
Fang, Yuan [3 ]
Zhang, Guoqing [1 ,2 ]
Xie, Guanlin [1 ,2 ]
Zhu, Bo [1 ,2 ]
Ibrahim, Muhammad [1 ,2 ]
机构
[1] Zhejiang Univ, State Key Lab Rice Biol, Minist Agr, Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China
[2] Zhejiang Univ, Key Lab Mol Biol Crop Pathogens & Insects, Minist Agr, Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China
[3] Zhejiang Normal Univ, Coll Chem & Life Sci, Hangzhou 310029, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
CYSTIC-FIBROSIS PATIENTS; SP-NOV; PSEUDOMONAS-CEPACIA; MAIZE RHIZOSPHERE; PCR TESTS; IDENTIFICATION; GENOMOVARS; BACTERIA; PROPOSAL; VIETNAMIENSIS;
D O I
10.1007/s00284-010-9758-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The purpose of this study was to determine the existence of Burkholderia cepacia complex (Bcc) at species level and the predominant species in the environment of moso bamboo plantations in Hangzhou, China. A total of 423 isolates were recovered from moso bamboo rhizhosphere soil samples of three sites on the selective medium during 2007-2008. Isolates were identified by Bcc-specific PCR assays, followed by recA-restriction fragment length polymorphism assays, species-specific PCR analysis, recA gene sequencing, multilocus sequence typing (MLST) scheme, and BOX-PCR fingerprinting for genomic diversity. Out of 423 isolates, 278 isolates were assigned to the following Bcc species, eight B. stabilis, 26 B. anthina, 193 B. pyrrocinia, and 51 B. arboris, which indicated B. pyrrocinia as the most dominant species followed by B. arboris. Moreover, false positives were observed in certain isolates of B. arboris while performing species-specific PCR test. Furthermore, the results of recA gene sequence similarity and MLST data demonstrated that nine isolates formed a single discrete cluster but were PCR negative to species-specific primers representing novel species may exist within the Bcc. In addition, BOX-PCR fingerprinting for all the Bcc isolates also showed the strain diversity. It is the first report of the existence of B. arboris and predominance of B. pyrrocinia in the moso bamboo environment.
引用
收藏
页码:650 / 658
页数:9
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