Partitioning of carbohydrates in salt-sensitive and salt-tolerant soybean callus cultures under salinity stress and its subsequent relief

Salt tolerant (R100) and sensitive (S100) cell lines of Glycine max (L.) that differ in their ability to accumulate sodium (Na) and chloride (Cl) under 100 mM salt stress, were used to compare the contribution of carbohydrates in osmotic adjustment. Calli were exposed to a 100 mM NaCl concentrations for 12 days followed by 16 days of relief from stress to determine the effect of salinity changes of sugar content in the two cell lines. The salt-tolerant and the salt-sensitive cell lines differed in the time at which, and the type of sugar, that increased during salt stress. However, recovery in sugar content parameters during relief from stress were similar in the two cell lines. The concentration of glucose and fructose increased at a rate closely corresponding to the increase in fresh weight, while the concentration of sucrose decreased to the control level coincident with relatively rapid growth.