Electrochemical studies of ganciclovir at boron-doped nanocrystalline diamond electrodes

The electrochemical oxidation of ganciclovir was investigated at boron-doped nanocrystalline diamond (BOND) electrodes by the use of cyclic voltammetry and differential pulse voltammetry. The optimization of the experimental variables including supporting electrolyte and pH value was studied, and the 0.04-M Britton-Robinson buffer solution (pH 2.5) was selected. The relationship of the oxidation peak potential to scan rate and pH value was also investigated, and 2 electron transfer and 2 proton participation for the oxidation process of ganciclovir at BOND electrode were obtained. Compared with boron-doped microcrystalline diamond and glassy carbon electrodes, the BDND electrode demonstrated the wider linear range of 0.5-350 mu M, lower limit of detection of 0.21 mu M, and higher reproducibility and stability for the determination of ganciclovir under the optimum conditions. For the analysis of ganciclovir in human serum at the BOND electrodes, precision and accuracy were checked by recovery experiments. (C) 2010 Elsevier B.V. All rights reserved.