Enhancing pathogen identification in patients with meningitis and a negative Gram stain using the BioFire FilmArray® Meningitis/Encephalitis panel

被引：54

作者：

Wootton, Susan H. ^{[1
]}

Aguilera, Elizabeth ^{[1
]}

Salazar, Lucrecia ^{[2
]}

Hemmert, Andrew C. ^{[3
]}

Hasbun, Rodrigo ^{[2
]}

机构：

[1] Univ Texas Hlth Sci Ctr Houston, Dept Pediat, Div Infect Dis, Houston, TX 77030 USA

[2] Univ Texas Hlth Sci Ctr Houston, Dept Internal Med, Div Infect Dis, 6431 Fannin St 2-112 MSB, Houston, TX 77030 USA

[3] BioFire Diagnost LLC, Salt Lake City, UT 84108 USA

来源：

ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS | 2016年 / 15卷

关键词：

Aseptic meningitis; Encephalitis; Molecular diagnostic techniques; Polymerase chain reaction; CENTRAL-NERVOUS-SYSTEM; INFECTIONS; DIAGNOSIS; VIRUS;

D O I：

10.1186/s12941-016-0137-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Meningitis with a negative cerebrospinal (CSF) Gram stain represents a diagnostic and therapeutic challenge. The purpose of our study was to evaluate the performance of the BioFire FilmArray (R) Meningitis/Encephalitis (FA ME) panel in patients presenting with community-acquired meningitis with a negative Gram stain. Methods: CSF from 48 patients with community-acquired meningitis with a negative Gram stain admitted to four hospitals in Houston, TX underwent additional testing by the FA ME. FA ME results were compared to results obtained as part of routine evaluation. Results: The panel detected pathogens not previously identified in 11 (22.9 %) of 48, but did not detect pathogens identified by standard technique (West Nile virus, Histoplasma) in 5 (15.2 %) patients. Conclusions: Rapid testing for the most common pathogens causing meningitis will aid in the diagnosis and treatment of patients with meningitis.

引用

页数：4

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