Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer

被引：29

作者：

Baker, Michelle ^{[1
,2
]}

Negus, David ^{[1
]}

Raghunathan, Dhaarini ^{[1
]}

Radford, Paul ^{[1
]}

Moore, Chris ^{[1
]}

Clark, Gemma ^{[3
]}

Diggle, Mathew ^{[3
]}

Tyson, Jess ^{[1
]}

Twycross, Jamie ^{[2
]}

Sockett, R. Elizabeth ^{[1
]}

机构：

[1] Univ Nottingham, Med Sch, Queens Med Ctr, Sch Life Sci, Nottingham NG7 2UH, England

[2] Univ Nottingham, Sch Comp Sci, Jubilee Campus,Wollaton Rd, Nottingham NG8 1BB, England

[3] Nottingham Univ Hosp NHS Trust, Queens Med Ctr, Empath Pathol Serv, Recept Floor A,West Block, Nottingham NG7 2UH, England

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

RESISTANCE; INFECTION; ANTIBIOTICS; PATHOGENS; ESKAPE; DECOYS;

D O I：

10.1038/s41598-017-08060-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as "living antibiotics" to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media. Here we combine mathematical modelling and lab experimentation to investigate the predation of an important carbapenem-resistant human pathogen, Klebsiella pneumoniae, by B. bacteriovorus in human serum versus buffer. We show experimentally that B. bacteriovorus is able to reduce prey numbers in each environment, on different timescales. Our mathematical model captures the underlying dynamics of the experimentation, including an initial predation-delay at the predator-preyserum interface. Our research shows differences between predation in buffer and serum and highlights both the potential and limitations of B. bacteriovorus acting therapeutically against K. pneumoniae in serum, informing future research into the medicinal behaviours and dosing of this living antibacterial.

引用

页数：18

共 46 条

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