Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus

被引：0

作者：

Tamai, Iradj Ashrafi ^{[1
,2
]}

Salehi, Taghi Zahraei ^{[3
]}

Sharifzadeh, Aghil ^{[4
]}

Shokri, Hojjatollah ^{[5
]}

Khosravi, Ali Reza ^{[4
]}

机构：

[1] Bu Ali Sina Univ, Hamadan, Iran

[2] Imam Khomeini Hosp, Res & Training Ctr, Tehran, Iran

[3] Univ Tehran, Dept Microbiol & Immunol, Fac Vet Med, Tehran, Iran

[4] Univ Tehran, Mycol Res Ctr, Fac Vet Med, Tehran, Iran

[5] Amol Univ Special Modern Technol, Fac Vet Med, Amol, Iran

来源：

Iranian Journal of Basic Medical Sciences | 2014年 / 17卷 / 11期

关键词：

Candida albicans; Candidiasis; Genotyping; HIV+; RPS sequence; 25S INTRON ANALYSIS; DUBLINIENSIS; EPIDEMIOLOGY; RPS;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Objective(s): Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV+) with or/and without oropharyngeal candidiasis (OPC). Materials and Methods: A total of 100 C. albicans isolates from Iranian HIV+ patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes. Results: The frequencies of genotypes A, B and C which were achieved using 25S rDNA, were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence. Conclusion: In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV+ patients.

引用

页码：831 / 835

页数：5

共 19 条

[1] Microsatellite polymorphism in the promoter sequence of the elongation factor 3 gene of Candida albicans as the basis for a typing system [J].