Longitudinal study of Salmonella spp., diarrheagenic Escherichia coli, Rotavirus, and Coronavirus isolated from healthy and diarrheic calves in a Brazilian dairy herd

被引：46

作者：

Coura, Fernanda Morcatti ^{[1
]}

Freitas, Moises Dias ^{[2
]}

Ribeiro, Juliane ^{[3
]}

de Leme, Raquel Arruda ^{[3
]}

de Souza, Cecilia ^{[3
]}

Alfieri, Amauri Alcindo ^{[3
]}

Facury Filho, Elias Jorge ^{[2
]}

de Carvalho, Antonio Ultimo ^{[2
]}

Silva, Marcos Xavier ^{[1
]}

Lage, Andrey Pereira ^{[1
]}

Heinemann, Marcos Bryan ^{[4
]}

机构：

[1] Univ Fed Minas Gerais, Escola Vet, Dept Med Vet Prevent, BR-31270010 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Escola Vet, Dept Clin & Cirurgia Vet, BR-31270010 Belo Horizonte, MG, Brazil

[3] Univ Estadual Londrina, Dept Med Vet Prevent, BR-86051990 Londrina, Parana, Brazil

[4] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, Brazil

来源：

TROPICAL ANIMAL HEALTH AND PRODUCTION | 2015年 / 47卷 / 01期

关键词：

Calf diarrhea; Coronavirus; Escherichia coli; Rotavirus; Salmonella spp; GROUP-A ROTAVIRUS; BOVINE CORONAVIRUS; VIRULENCE FACTORS; CALF DIARRHEA; MULTIPLEX PCR; CONCURRENT INFECTIONS; NEONATAL CALVES; NESTED PCR; PREVALENCE; CATTLE;

D O I：

10.1007/s11250-014-0675-5

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This prospective longitudinal study investigated the epidemiology of enteric disease associated with infections in calves aging up to 70 days. A total of 850 fecal samples were collected from 67 calves. Seventeen isolates of Salmonella spp. were recovered from feces of 11 calves (16.4 %), and statistical analysis revealed no association between the presence of Salmonella spp. and clinical signs of diarrhea or age. Virulence factors of Escherichia coli were identified in 103 strains: eae (7), K99/STa (7), Stx1 (7), Stx1/eae (36), Stx1/Stx2/eae (2), Stx2 (43), and Stx2/eae (1). There was statistical association between diarrheic animals carrying E. coli Stx1/eae (+) in their feces at 2 and 4 weeks of age (P = 0.003) and E. coli Stx2 (+) at 5 weeks of age (P = 0.03). Rotavirus was detected in 49 (5.76 %) fecal samples collected from 33 calves (49.2 %). The presence of rotavirus was correlated with diarrheic feces (P < 0.0001) rather than feces with normal consistency. There was a significant relationship between age group and diarrhea (P = 0.001). Bovine coronavirus (BCoV) was detected in 93 fecal samples collected from 46 calves (68.6 %). There was an association (P < 0.0001) between diarrheic animals positive for BCoV and age groups. The results demonstrate the importance of the pathogens studied in the etiology of diarrhea in calves.

引用

页码：3 / 11

页数：9

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