Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2

被引：57

作者：

Kocic, Jelena ^{[1
]}

Santibanez, Juan F. ^{[1
]}

Krstic, Aleksandra ^{[1
]}

Mojsilovic, Slavko ^{[1
]}

Dordevic, Ivana Okic ^{[1
]}

Trivanovic, Drenka ^{[1
]}

Ilic, Vesna ^{[2
]}

Bugarski, Diana ^{[1
]}

机构：

[1] Univ Belgrade, Inst Med Res, Lab Expt Hematol & Stem Cells, Belgrade 11129, Serbia

[2] Univ Belgrade, Inst Med Res, Immunol Lab, Belgrade 11129, Serbia

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2012年 / 1823卷 / 04期

关键词：

C2C12; Myoblast; IL-17; Myogenic differentiation; Osteogenic differentiation; ERK1,2; MESENCHYMAL STEM-CELLS; PROTEIN-KINASE; HEMATOPOIETIC-CELLS; SIGNALING PATHWAY; CYTOKINE RELEASE; STROMAL CELLS; MUSCLE-CELLS; T-CELLS; EXPRESSION; IL-17;

D O I：

10.1016/j.bbamcr.2012.01.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study evaluated the role of interleukin (IL) 17 in multilineage commitment of C2C12 myoblastic cells and investigated associated signaling pathways. The results concerning the effects on cell function showed that IL-17 inhibits the migration of C2C12 cells, while not affecting their proliferation. The data regarding the influence on differentiation demonstrated that IL-17 inhibits myogenic differentiation of C2C12 cells by down-regulating the myogenin mRNA level, myosin heavy chain expression and myotube formation, but promotes their osteogenic differentiation by up-regulating the Runt-related transcription factor 2 mRNA level, cyclooxygenase-2 expression and alkaline phosphatase activity. IL-17 exerted these effects by activating ERK1,2 mitogen activated protein kinase signaling pathway, which in turn regulated the expression of relevant genes and proteins to inhibit myogenic differentiation and induce osteogenic differentiation. Additional analysis showed that the induction of osteogenic differentiation by IL-17 is independent of BMP signaling. The results obtained demonstrate the potential of IL-17 not only to inhibit the myogenic differentiation of C2C12 myoblasts but also to convert their differentiation pathway into that of osteoblast lineage providing new insight into the capacities of IL-17 to modulate the differentiation commitment. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：838 / 849

页数：12

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