Enzyme linking to DNA aptamers via a zinc finger as a bridge

被引：6

作者：

Abe, Koichi ^{[1
]}

Murakami, Yoshiyuki ^{[1
]}

Tatsumi, Atsuro ^{[1
]}

Sumida, Koichi ^{[1
]}

Kezuka, Aki ^{[1
]}

Fukaya, Takahiro ^{[1
]}

Kumagai, Takenori ^{[1
]}

Osawa, Yuko ^{[1
]}

Sode, Koji ^{[1
]}

Ikebukuro, Kazunori ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 57期

关键词：

ELECTROCHEMICAL DETECTION; HUMAN THROMBIN; GROWTH-FACTOR; PROTEIN; SELECTION; SANDWICH; RECOGNITION; BINDING; ASSAY; PAIRS;

D O I：

10.1039/c5cc02906f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We propose a novel enzyme-labeling method for DNA aptamers using enzyme-fused zinc finger proteins. We achieved thrombin detection and vascular endothelial growth factor detection using zinc finger-fused firefly luciferase.

引用

页码：11467 / 11469

页数：3

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