Analgesic and anti-inflammatory effects of the amphibian neurotoxin, anntoxin

被引：20

作者：

Wei, Lin ^{[1
]}

Dong, Li ^{[2
,3
]}

Zhao, Tongyan ^{[4
]}

You, Dewen ^{[2
]}

Liu, Rui ^{[1
]}

Hu, Huan ^{[2
]}

Yang, Hailong ^{[2
]}

Lai, Ren ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Life Sci Coll, Minist Agr, Key Lab Microbiol Engn Agr Environm, Nanjing 210095, Jiangsu, Peoples R China

[2] Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing 100009, Peoples R China

[4] Acad Mil Med Sci, Beijing Inst Microbiol & Epidemiol, Beijing, Peoples R China

来源：

BIOCHIMIE | 2011年 / 93卷 / 06期

关键词：

Amphibian; Skin; Neurotoxin; Analgesic; Anti-inflammation; GATED SODIUM-CHANNELS; TOAD BOMBINA-MAXIMA; ANTIMICROBIAL PEPTIDES; SKIN SECRETIONS; LEAF EXTRACT; PAIN; PRECURSOR; MODELS; RATS; MICE;

D O I：

10.1016/j.biochi.2011.02.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anntoxin is the first gene-encoded neurotoxin identified from amphibians, which is a 60-residue neurotoxin peptide, acting as an inhibitor of tetrodotoxin-sensitive (TTX-S) voltage-gated sodium channel (VGSC). Sodium channels have been considered as therapeutic targets for pain. Several animal models of persistent inflammatory and neuropathic pain (tail-flick test, hot plate test, acetic acid-induced writhing test, formalin-induced paw licking, carrageenan-induced paw edema) were used to test analgesic functions of recombinant anntoxin (r-anntoxin). In all these animal models, r-anntoxin showed strong analgesic functions. R-anntoxin obviously inhibited secretions of both tumor necrosis factor alpha (TNF-alpha) and cyclooxygenase-2 (COX-2). Histopathological study indicated that r-anntoxin reduced the edematous epidermis induced by carrageenan. All these results indicate that r-anntoxin has strong analgesic and anti-inflammatory activities. (C) 2011 Elsevier Masson SAS. All rights reserved.

引用

页码：995 / 1000

页数：6

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