Molecular Phylogeny of Burkholderia pseudomallei from a Remote Region of Papua New Guinea

被引:18
作者
Baker, Anthony [1 ]
Pearson, Talima [2 ]
Price, Erin P. [2 ]
Dale, Julia [2 ]
Keim, Paul [2 ]
Hornstra, Heidie [2 ]
Greenhill, Andrew [1 ,3 ]
Padilla, Gabriel [1 ]
Warner, Jeffrey [1 ]
机构
[1] James Cook Univ, Sch Vet & Biomed Sci, Environm & Publ Hlth Microbiol Res Grp, Townsville, Qld 4811, Australia
[2] No Arizona Univ, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA
[3] Papua New Guinea Inst Med Res, Goroka, Papua N Guinea
关键词
PSEUDOMONAS-PSEUDOMALLEI; GENETIC DIVERSITY; AUSTRALIA; MELIOIDOSIS; MAXIMUM;
D O I
10.1371/journal.pone.0018343
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: The island of New Guinea is located midway between the world's two major melioidosis endemic regions of Australia and Southeast Asia. Previous studies in Papua New Guinea have demonstrated autochthonous melioidosis in Balimo, Western province. In contrast to other regions of endemicity, isolates recovered from both environmental and clinical sources demonstrate narrow genetic diversity over large spatial and temporal scales. Methodology/Principal Findings: We employed molecular typing techniques to determine the phylogenetic relationships of these isolates to each other and to others worldwide to aid in understanding the origins of the Papua New Guinean isolates. Multi-locus sequence typing of the 39 isolates resolved three unique sequence types. Phylogenetic reconstruction and Structure analysis determined that all isolates were genetically closer to those from Australia than those from Southeast Asia. Gene cluster analysis however, identified a Yersinia-like fimbrial gene cluster predominantly found among Burkholderia pseudomallei derived from Southeast Asia. Higher resolution VNTR typing and phylogenetic reconstruction of the Balimo isolates resolved 24 genotypes with long branch lengths. These findings are congruent with long term persistence in the region and a high level of environmental stability. Conclusions/Significance: Given that anthropogenic influence has been hypothesized as a mechanism for the dispersal of B. pseudomallei, these findings correlate with limited movement of the indigenous people in the region. The palaeogeographical and anthropogenic history of Australasia and the results from this study indicate that New Guinea is an important region for the further study of B. pseudomallei origins and dissemination.
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页数:6
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