The Antisenescence Effect of Exosomes from Human Adipose-Derived Stem Cells on Skin Fibroblasts

被引：16
|
作者：
Guo, Ji-An [1 ]
Yu, Pi-Jun [1 ]
Yang, Dong-Qin [2 ]
Chen, Wei [1 ]
机构：
[1] Shanghai Eighth Peoples Hosp, Dept Aesthet Plast Surg, Shanghai 200235, Peoples R China
[2] Shanghai Eighth Peoples Hosp, Dept Hematol, Shanghai 200235, Peoples R China
来源：
BIOMED RESEARCH INTERNATIONAL | 2022年 / 2022卷
关键词：
EXTRACELLULAR VESICLES; SENESCENCE; PARADIGM; ENHANCE;
D O I：
10.1155/2022/1034316
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Human adipose-derived stem cells (ADSCs) have become a promising therapeutic approach against skin aging. Recent studies confirm that exosomes partially mediate the therapeutic effect of stem cells. This study successfully isolated exosomes from the ADSC culture medium and discovered that ADSC-derived exosomes (ADSC-Exos) could alleviate human dermal fibroblast (HDF) senescence and stimulate HDF migration. Moreover, ADSC-Exos increased the type I collagen expression level and reduced the reactive oxygen species (ROS) and senescence-associated beta-galactosidase (SA-beta-Gal) activity in HDFs. In addition, we demonstrated that ADSC-Exos significantly inhibited senescence-related protein expression levels of p53, p21, and p16. In conclusion, our results have revealed the antisenescence effects of ADSC-Exos on HDFs and ADSC-Exos may be a novel cell-free therapeutic tool for antiaging.
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页数：12
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