DNA vaccination against respiratory influenza virus infection

被引：22
作者：
Wong, JP
Zabielski, MA
Schmaltz, FL
Brownlee, GG
Bussey, LA
Marshall, K
Borralho, T
Nagata, LP
机构：
[1] Def Res Estab Suffield, Chem & Biol Def Sect, Medicine Hat, AB T1A 8K6, Canada
[2] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
来源：
VACCINE | 2001年 / 19卷 / 17-19期
关键词：
DNA vaccine; influenza; liposomes; immunity;
D O I：
10.1016/S0264-410X(00)00474-6
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
DNA vaccination using plasmid encoding the hemagglutinin (HA) gene of influenza A/PR, 8/34 virus to induct long-lasting protective immunity against respiratory infection was evaluated in this study. Using liposomes as carriers, the efficacy of DNA vaccines was determined using a lethal influenza infection model in mice. Mice immunized intranasally or intramuscularly with liposome-encapsulated pCl plasmid encoding HA (pCl-HA10) were completely protected against an intranasal 5 LD50 influenza virus challenge. Mice immunized with liposome-encapsulated pCl-HA10, but not naked pCl-HA10, by intranasal administration were found to product high titers of serum IEA. These results suggest DNA vaccines encapsulated in liposomes are efficacious in inducing complete protective immunity against respiratory influenza virus infection. Crown Copyright (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：2461 / 2467
页数：7
相关论文
共 13 条
[1]  
*CDC, 1992, MMWR-MORBID MORTAL W, V52, P1
[2]   BOVINE HERPESVIRUS-1 - IMMUNE-RESPONSES IN MICE AND CATTLE INJECTED WITH PLASMID DNA [J].
COX, GJM ;
ZAMB, TJ ;
BABIUK, LA .
JOURNAL OF VIROLOGY, 1993, 67 (09) :5664-5667
[3]   Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells [J].
Doe, B ;
Selby, M ;
Barnett, S ;
Baenziger, J ;
Walker, CM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (16) :8578-8583
[4]   DNA vaccines [J].
Donnelly, JJ ;
Ulmer, JB ;
Shiver, JW ;
Liu, MA .
ANNUAL REVIEW OF IMMUNOLOGY, 1997, 15 :617-648
[5]   Construction and characterization of a novel recombinant single-chain variable fragment antibody against western equine encephalitis virus [J].
Long, MC ;
Jager, S ;
Mah, DCW ;
Jebailey, L ;
Mah, MA ;
Masri, SA ;
Nagata, LP .
HYBRIDOMA, 2000, 19 (01) :1-13
[6]   Biodistribution and gene expression of lipid/plasmid complexes after systemic administration [J].
Mahato, RI ;
Anwer, K ;
Tagliaferri, F ;
Meaney, G ;
Leonard, P ;
Wadhwa, MS ;
Logan, M ;
French, M ;
Rolland, A .
HUMAN GENE THERAPY, 1998, 9 (14) :2083-2099
[7]  
McCluskie MJ, 1999, CRIT REV IMMUNOL, V19, P303
[8]   EXPOSING THE IMMUNOLOGY OF NAKED DNA VACCINES [J].
PARDOLL, DM ;
BECKERLEG, AM .
IMMUNITY, 1995, 3 (02) :165-169
[9]   A BACTERIOPHAGE-T7 RNA-POLYMERASE PROMOTER SYSTEM FOR CONTROLLED EXCLUSIVE EXPRESSION OF SPECIFIC GENES [J].
TABOR, S ;
RICHARDSON, CC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (04) :1074-1078
[10]   CYTO-TOXIC T-CELL RECOGNITION OF THE INFLUENZA NUCLEOPROTEIN AND HEMAGGLUTININ EXPRESSED IN TRANSFECTED MOUSE L-CELLS [J].
TOWNSEND, ARM ;
MCMICHAEL, AJ ;
CARTER, NP ;
HUDDLESTON, JA ;
BROWNLEE, GG .
CELL, 1984, 39 (01) :13-25
12