1H nuclear magnetic resonance based metabolic urinary profiling of patients with ischemic heart failure

被引:73
作者
Kang, Seok-Min [2 ,3 ]
Park, Jong-Chul [1 ,4 ]
Shin, Min-Jeong [5 ]
Lee, Hyeran [5 ]
Oh, Jaewon [2 ,3 ]
Ryu, Do Hyun [6 ]
Hwang, Geum-Sook [1 ,4 ]
Chung, Ji Hyung [2 ,3 ,7 ]
机构
[1] Korea Basic Sci Inst, Seoul 136713, South Korea
[2] Yonsei Univ, Coll Med, Div Cardiol, Yonsei Cardiovasc Hosp, Seoul 120752, South Korea
[3] Yonsei Univ, Coll Med, Cardiovasc Res Inst, Seoul 120752, South Korea
[4] Chungnam Natl Univ, Grad Sch Analyt Sci & Technol, Taejon 305764, South Korea
[5] Korea Univ, Dept Food & Nutr, Seoul 136703, South Korea
[6] Sungkyunkwan Univ, Dept Chem, Gyeonggi Do 440746, South Korea
[7] Yonsei Univ Hlth Syst, Severance Integrat Res Inst Cerebral & Cardiovasc, Seoul 120752, South Korea
关键词
Heart failure; Citric acid cycle; Metabolism; Methylmalonic acid; Phenylacetylglycine; MODIFIED NUCLEOSIDES; PEROXISOME PROLIFERATION; MYOCARDIAL-INFARCTION; PLASMA HOMOCYSTEINE; GENE-EXPRESSION; CANCER PATIENTS; FAILING HEART; SERUM; REVEALS; DISEASE;
D O I
10.1016/j.clinbiochem.2010.11.010
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objectives: We sought to identify metabolic pathways characterizing human heart failure (HF) using (NMR)-N-1 based urinary metabolomic analysis in conjunction with multivariate statistics. Design and methods: Patients with systolic HF of ischemic origin (n = 15) and healthy controls (n = 20) participated in this study. Patients with type 2 diabetes mellitus were excluded. Results: The results showed that the urine of the HF patients had higher levels of metabolites for acetate (p<0.05) and acetone (p<0.01) compared to the healthy controls. In addition, there was a perturbation in methylmalonate metabolism as shown by increased urinary levels of methylmalonic acid (p<0.001) in the HF patients. HF patients also had increased urinary levels of cytosine (p<0.01) and phenylacetylglycine (p<0.01) and decreased 1-methylnicotinamide (p<0.05) compared to healthy controls. Conclusions: TCA cycle metabolites and fatty acid metabolism were modified in the HF patients, indicating altered energy metabolism. Moreover, perturbations of metabolism in nucleotide and methylmalonate were observed. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:293 / 299
页数:7
相关论文
共 34 条
[1]   Inflammatory mediators in chronic heart failure: An overview [J].
Anker, SD ;
von Haehling, S .
HEART, 2004, 90 (04) :464-470
[2]   Global Gene Expression Profiling in the Failing Myocardium [J].
Asakura, Masanori ;
Kitakaze, Masafumi .
CIRCULATION JOURNAL, 2009, 73 (09) :1568-1576
[3]   Metabolomic profiling to develop blood biomarkers for Parkinson's disease [J].
Bogdanov, Mikhail ;
Matson, Wayne R. ;
Wang, Lei ;
Matson, Theodore ;
Saunders-Pullman, Rachel ;
Bressman, Susan S. ;
Beal, M. Flint .
BRAIN, 2008, 131 :389-396
[4]   Anaplerotic molecules: Current and future [J].
Brunengraber, Henri ;
Roe, Charles R. .
JOURNAL OF INHERITED METABOLIC DISEASE, 2006, 29 (2-3) :327-331
[5]   Development of a multivariate statistical model to predict peroxisome proliferation in the rat, based on urinary 1H-NMR spectral patterns [J].
Connor, SC ;
Hodson, MP ;
Ringeissen, S ;
Sweatman, BC ;
McGill, PJ ;
Waterfield, CJ ;
Haselden, JN .
BIOMARKERS, 2004, 9 (4-5) :364-385
[6]   Serum metabolomics reveals many novel metabolic markers of heart failure, including pseudouridine and 2-oxoglutarate [J].
Dunn, Warwick B. ;
Broadhurst, David I. ;
Deepak, Sasalu M. ;
Buch, Mamta H. ;
McDowell, Garry ;
Spasic, Irena ;
Ellis, David I. ;
Brooks, Nicholas ;
Kell, Douglas B. ;
Neyses, Ludwig .
METABOLOMICS, 2007, 3 (04) :413-426
[7]   Application of 1H NMR-based metabonomics in the study of metabolic profiling of human hepatocellular carcinoma and liver cirrhosis [J].
Gao, Hongchang ;
Lu, Qiang ;
Liu, Xia ;
Cong, Hui ;
Zhao, Liangcai ;
Wang, Huimin ;
Lin, Donghai .
CANCER SCIENCE, 2009, 100 (04) :782-785
[8]   The generalization of Student's ratio [J].
Hotelling, H .
ANNALS OF MATHEMATICAL STATISTICS, 1931, 2 :360-378
[9]  
Jacques PF, 2001, AM J CLIN NUTR, V73, P613
[10]   Metabolomics: A Global Biochemical Approach to the Study of Central Nervous System Diseases [J].
Kaddurah-Daouk, Rima ;
Krishnan, K. Ranga Rama .
NEUROPSYCHOPHARMACOLOGY, 2009, 34 (01) :173-186