Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-D-rhamnose (D-acofriose)

被引：13

作者：

Boyko, Alevtina S. ^{[1
]}

Dmitrenok, Andrey S. ^{[2
]}

Fedonenko, Yuliya P. ^{[1
]}

Zdorovenko, Evelina L. ^{[2
]}

Konnova, Svetlana A. ^{[1
,3
]}

Knirel, Yuriy A. ^{[2
]}

Ignatov, Vladimir V. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia

[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

[3] Chernyshevsky Saratov State Univ, Saratov 410012, Russia

来源：

CARBOHYDRATE RESEARCH | 2012年 / 355卷

基金：

俄罗斯基础研究基金会;

关键词：

Lipopolysaccharide; O-Antigen; Bacterial polysaccharide structure; D-Acofriose; Azospirillum brasilense; PLANT-GROWTH; MONOSACCHARIDES;

D O I：

10.1016/j.carres.2012.04.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two types of neutral O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide isolated by phenol-water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense Jm6B2. The following structure of the major O-polysaccharide was established by composition and methylation (ethylation) analyses, Smith degradation, and 1D and 2D H-1 and C-13 NMR spectroscopy: [GRAPHICS] where a non-stoichiometric (similar to 60%) 3-O-methylation of D-rhamnose is indicated by italics. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：92 / 95

页数：4

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