Severe community-acquired pneumonia caused by Chlamydia psittaci genotype E/B strain circulating among geese in Lishui city, Zhejiang province, China

被引:10
作者
Qin, Xin-Cheng [1 ]
Huang, Jinwei [2 ]
Yang, Zhangnv [3 ]
Sun, Xiangrong [4 ]
Wang, Wen [1 ]
Gong, Enhui [2 ]
Cao, Zhuo [2 ]
Lin, Jianfeng [5 ]
Qiu, Yanai [5 ]
Wen, Bohai [6 ]
Kan, Biao [1 ]
Xu, Jianguo [1 ]
Qin, Tian [1 ]
机构
[1] Chinese Ctr Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Di, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, 155 Changbai Rd, Beijing 102206, Peoples R China
[2] Wenzhou Med Univ, Dept Resp & Crit Care Med, Affiliated Hosp 6, Lishui, Peoples R China
[3] Zhejiang Prov Ctr Dis Control & Prevent, Key Lab Vaccine Prevent & Control Infect Dis Zhej, Hangzhou, Peoples R China
[4] Nanchang Ctr Dis Control & Prevent, Dept Clin Lab, Nanchang, Jiangxi, Peoples R China
[5] Suichang Hosp Tradit Chinese Med, Dept Internal Med, Lishui, Peoples R China
[6] Beijing Inst Microbiol & Epidemiol, Pathogen & Biosecur, Beijing, Peoples R China
基金
中国国家自然科学基金;
关键词
Community-acquired pneumonia; psittacosis; Chlamydia psittaci; poultry; zoonotic potential;
D O I
10.1080/22221751.2022.2140606
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Between November 2021 and January 2022, four patients of community-acquired pneumonia were admitted to the hospitals in Lishui city, Zhejiang province, China. Their main clinical manifestations were fever and dry cough as well as radiographic infiltrate, but the empiric antimicrobial therapy or traditional Chinese medicine was not effective for their illness. Clinical specimens from the patients as well as environmental and poultry specimens were collected for the determination of the causative pathogen. The ompA gene and seven housekeeping genes of Chlamydia psittaci were successfully amplified from all the patients, and the sequences of each gene were identical to one another, suggesting that they were infected by the same strain of C. psittaci. A novel strain of C. psittaci (LS strain) was isolated from the bronchoalveolar lavage fluid of patient 2 and its whole genome was obtained. Phylogenetic analyses based on the whole-genome sequences showed that the isolate is most closely related to the strain (WS/RT/E30) identified as genotype E/B. In addition, The ompA gene and four housekeeping genes of C. psittaci were also amplified from two of four faeces samples of geese at the home of patient 2, and the sequences from geese were 100% identical to those from the patients. Accordingly, these cases could be attributed to a circulating C. psittaci strain of genotype E/B in the local geese. Therefore, there is an urgent need to strengthen the regional surveillance on C. psittaci among poultry and humans for prevention and control of the outbreak of psittacosis in the city.
引用
收藏
页码:2715 / 2723
页数:9
相关论文
共 16 条
[1]   Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms [J].
Everett, KDE ;
Bush, RM ;
Andersen, AA .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :415-440
[2]   Sequencing of the Chlamydophila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method [J].
Geens, T ;
Desplanques, A ;
Van Loock, M ;
Bönner, BM ;
Kaleta, EF ;
Magnino, S ;
Andersen, AA ;
Everett, KDE ;
Vanrompay, D .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (05) :2456-2461
[3]   From genomes to genotypes: molecular epidemiological analysis of Chlamydia gallinacea reveals a high level of genetic diversity for this newly emerging chlamydial pathogen [J].
Guo, Weina ;
Jelocnik, Martina ;
Li, Jing ;
Sachse, Konrad ;
Polkinghorne, Adam ;
Pannekoek, Yvonne ;
Kaltenboeck, Bernhard ;
Gong, Jiansen ;
You, Jinfeng ;
Wang, Chengming .
BMC GENOMICS, 2017, 18
[4]   Chlamydophila psittaci genotype E/B transmission from African grey parrots to humans [J].
Harkinezhad, Taher ;
Verminnen, Kristel ;
Van Droogenbroeck, Caroline ;
Vanrompay, Daisy .
JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 56 (08) :1097-1100
[5]   Community-Acquired Pneumonia Requiring Hospitalization among US Adults [J].
Jain, S. ;
Self, W. H. ;
Wunderink, R. G. ;
Fakhran, S. ;
Balk, R. ;
Bramley, A. M. ;
Reed, C. ;
Grijalva, C. G. ;
Anderson, E. J. ;
Courtney, D. M. ;
Chappell, J. D. ;
Qi, C. ;
Hart, E. M. ;
Carroll, F. ;
Trabue, C. ;
Donnelly, H. K. ;
Williams, D. J. ;
Zhu, Y. ;
Arnold, S. R. ;
Ampofo, K. ;
Waterer, G. W. ;
Levine, M. ;
Lindstrom, S. ;
Winchell, J. M. ;
Katz, J. M. ;
Erdman, D. ;
Schneider, E. ;
Hicks, L. A. ;
McCullers, J. A. ;
Pavia, A. T. ;
Edwards, K. M. ;
Finelli, L. .
NEW ENGLAND JOURNAL OF MEDICINE, 2015, 373 (05) :415-427
[6]   Zoonotic infection with Chlamydia psittaci at an avian refuge centre [J].
Kalmar, Isabelle D. ;
Dicxk, Veerle ;
Dossche, Liesbeth ;
Vanrompay, Daisy .
VETERINARY JOURNAL, 2014, 199 (02) :300-302
[7]   A parrot-type Chlamydia psittaci strain is in association with egg production drop in laying ducks [J].
Lin, Wencheng ;
Chen, Tong ;
Liao, Liqin ;
Wang, Zhanxin ;
Xiao, Junfang ;
Lu, Junpeng ;
Song, Chuncheng ;
Qin, Jianping ;
Chen, Feng ;
Chang, Yung-Fu ;
Xie, Qingmei .
TRANSBOUNDARY AND EMERGING DISEASES, 2019, 66 (05) :2002-2010
[8]   THE MORPHOLOGY OF CHLAMYDIA-PNEUMONIAE [J].
MIYASHITA, N ;
KANAMOTO, Y ;
MATSUMOTO, A .
JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 38 (06) :418-425
[9]   Multi Locus Sequence Typing of Chlamydia Reveals an Association between Chlamydia psittaci Genotypes and Host Species [J].
Pannekoek, Yvonne ;
Dickx, Veerle ;
Beeckman, Delphine S. A. ;
Jolley, Keith A. ;
Keijzers, Wendy C. ;
Vretou, Evangelia ;
Maiden, Martin C. J. ;
Vanrompay, Daisy ;
van der Ende, Arie .
PLOS ONE, 2010, 5 (12)
[10]   New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples [J].
Pantchev, Alexandra ;
Sting, Reinhard ;
Bauerfeind, Rolf ;
Tyczka, Judith ;
Sachse, Konrad .
VETERINARY JOURNAL, 2009, 181 (02) :145-150