Low-intensity pulsed ultrasound regulates proliferation and differentiation of osteoblasts through osteocytes

被引：50
作者：
Li, Lei [1 ]
Yang, Zheng [1 ]
Zhang, Hai [2 ]
Chen, Wenchuan [1 ]
Chen, Mengshi [3 ]
Zhu, Zhimin [1 ]
机构：
[1] Sichuan Univ, W China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China
[2] Univ Washington, Sch Dent, Dept Restorat Dent, Seattle, WA 98195 USA
[3] Sichuan Univ, Dept Biomech, Chengdu 610041, Sichuan, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 418卷 / 02期
关键词：
Ultrasound; Osteoblast; Osteocyte; Nitric oxide (NO); Prostaglandin E-2 (PGE(2)); NITRIC-OXIDE; GROWTH-FACTOR; PROSTAGLANDIN E-2; BONE-FORMATION; TGF-BETA; CELLS; PROTEIN; MINERALIZATION; RESPONSES; STRAIN;
D O I：
10.1016/j.bbrc.2012.01.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Low-intensity pulsed ultrasound (LIPUS) has been used as a safe and effective modality to enhance fracture healing. As the most abundant cells in bone, osteocytes orchestrate biological activities of effector cells via direct cell-to-cell contacts and by soluble factors. In this study, we have used the osteocytic MLO-Y4 cells to study the effects of conditioned medium from LIPUS-stimulated MLO-Y4 cells on proliferation and differentiation of osteoblastic MC3T3-E1 cells. Conditioned media from LIPUS-stimulated MLO-Y4 cells (LIPUS-Osteocyte-CM) were collected and added on MC3T3-E1 cell cultures. MC3T3-E1 cells cultured in LIPUS-Osteocyte-CM demonstrated a significant inhibition of proliferation and an increased alkaline phosphatase activity. The results of PGE(2) and NO assay showed that LIPUS could enhance PGE(2) and NO secretion from MLO-Y4 cells at all time points within 24 h after LIPUS stimulation. We conclude that LIPUS regulates proliferation and differentiation of osteoblasts through osteocytes in vitro. Increased secretion of PGE(2) from osteocytes may play a role in this effect. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码：296 / 300
页数：5
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