Diversity in the Enteric Viruses Detected in Outbreaks of Gastroenteritis from Mumbai, Western India

被引:51
作者
Chitambar, Shobha [1 ]
Gopalkrishna, Varanasi [1 ]
Chhabra, Preeti [1 ]
Patil, Pooja [1 ]
Verma, Harsha [1 ]
Lahon, Anismrita [1 ]
Arora, Ritu [1 ]
Tatte, Vaishali [1 ]
Ranshing, Sujata [1 ]
Dhale, Ganesh [1 ]
Kolhapure, Rajendra [1 ]
Tikute, Sanjay [1 ]
Kulkarni, Jagannath [2 ]
Bhardwaj, Renu [3 ]
Akarte, Sulbha [4 ]
Pawar, Sashikant [4 ]
机构
[1] Natl Inst Virol, Enter Viruses Grp, Pune 411001, Maharashtra, India
[2] Gokuldas Tejpal Hosp, Dept Pathol, Bombay 400001, Maharashtra, India
[3] Sir Jamshedjee Jeejeebhoy Hosp, Dept Microbiol, Bombay 400008, Maharashtra, India
[4] Sir Jamshedjee Jeejeebhoy Hosp, Dept Prevent & Social Med, Bombay 400008, Maharashtra, India
关键词
adenovirus; Aichivirus; astrovirus; enterovirus; norovirus; rotavirus; gastroenteritis outbreak; GROUP-B ROTAVIRUS; REVERSE TRANSCRIPTION-PCR; FOODBORNE OUTBREAK; AICHI VIRUS; IDENTIFICATION; INFECTIONS; ADENOVIRUSES; CHILDREN; DIARRHEA; STRAINS;
D O I
10.3390/ijerph9030895
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Faecal specimens collected from two outbreaks of acute gastroenteritis that occurred in southern Mumbai, India in March and October, 2006 were tested for seven different enteric viruses. Among the 218 specimens tested, 95 (43.6%) were positive, 73 (76.8%) for a single virus and 22 (23.2%) for multiple viruses. Single viral infections in both, March and October showed predominance of enterovirus (EV, 33.3% and 40%) and rotavirus A (RVA, 33.3% and 25%). The other viruses detected in these months were norovirus (NoV, 12.1% and 10%), rotavirus B (RVB, 12.1% and 10%), enteric adenovirus (AdV, 6.1% and 7.5%), Aichivirus (AiV, 3% and 7.5%) and human astrovirus (HAstV, 3% and 0%). Mixed viral infections were largely represented by two viruses (84.6% and 88.9%), a small proportion showed presence of three (7.7% and 11%) and four (7.7% and 0%) viruses in the two outbreaks. Genotyping of the viruses revealed predominance of RVA G2P[4], RVB G2 (Indian Bangladeshi lineage), NoV GII.4, AdV-40, HAstV-8 and AiV B types. VP1/2A junction region based genotyping showed presence of 11 different serotypes of EVs. Although no virus was detected in the tested water samples, examination of both water and sewage pipelines in gastroenteritis affected localities indicated leakages and possibility of contamination of drinking water with sewage water. Coexistence of multiple enteric viruses during the two outbreaks of gastroenteritis emphasizes the need to expand such investigations to other parts of India.
引用
收藏
页码:895 / 915
页数:21
相关论文
共 41 条
[31]   Enteroviruses in Patients with Acute Encephalitis, Uttar Pradesh, India [J].
Sapkal, Gajanan N. ;
Bondre, Vijay P. ;
Fulmali, Pradip V. ;
Patil, Pooja ;
Gopalkrishna, V. ;
Dadhania, Vipul ;
Ayachit, Vijay M. ;
Gangale, Daya ;
Kushwaha, K. P. ;
Rathi, A. K. ;
Chitambar, Shobha D. ;
Mishra, Akhilesh Chandra ;
Gore, Milind M. .
EMERGING INFECTIOUS DISEASES, 2009, 15 (02) :295-298
[32]   Picornavirus Salivirus/Klassevirus in Children with Diarrhea, China [J].
Shan, Tongling ;
Wang, Chunmei ;
Cui, Li ;
Yu, Ying ;
Delwart, Eric ;
Zhao, Wei ;
Zhu, Caixia ;
Lan, Daoliang ;
Dai, Xiuqiang ;
Hua, Xiuguo .
EMERGING INFECTIOUS DISEASES, 2010, 16 (08) :1303-1305
[33]   MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0 [J].
Tamura, Koichiro ;
Dudley, Joel ;
Nei, Masatoshi ;
Kumar, Sudhir .
MOLECULAR BIOLOGY AND EVOLUTION, 2007, 24 (08) :1596-1599
[34]   Characterization of Group A Rotavirus Infections in Adolescents and Adults From Pune, India: 1993-1996 and 2004-2007 [J].
Tatte, Vaishali S. ;
Gentsch, Jon R. ;
Chitambar, Shobha D. .
JOURNAL OF MEDICAL VIROLOGY, 2010, 82 (03) :519-527
[35]   Identification and Characterization of Enteric Adenoviruses in Infants and Children Hospitalized for Acute Gastroenteritis [J].
Verma, Harsha ;
Chitambar, Shobha D. ;
Varanasi, Gopalkrishna .
JOURNAL OF MEDICAL VIROLOGY, 2009, 81 (01) :60-64
[36]   Astrovirus associated acute gastroenteritis in western India: Predominance of dual serotype strains [J].
Verma, Harsha ;
Chitambar, Shobha D. ;
Gopalkrishna, V. .
INFECTION GENETICS AND EVOLUTION, 2010, 10 (04) :575-579
[37]   A large infantile gastroenteritis outbreak in Albania caused by multiple emerging rotavirus genotypes [J].
Villena, C ;
Gabrieli, R ;
Pintó, RM ;
Guix, S ;
Donia, D ;
Buonomo, E ;
Palombi, L ;
Cenko, F ;
Bino, S ;
Bosch, A ;
Divizia, M .
EPIDEMIOLOGY AND INFECTION, 2003, 131 (03) :1105-1110
[38]   REMOVAL OF INHIBITORY SUBSTANCES FROM HUMAN FECAL SPECIMENS FOR DETECTION OF GROUP-A ROTAVIRUSES BY REVERSE-TRANSCRIPTASE AND POLYMERASE CHAIN REACTIONS [J].
WILDE, J ;
EIDEN, J ;
YOLKEN, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (06) :1300-1307
[39]   Viruses causing gastroenteritis [J].
Wilhelmi, I ;
Roman, E ;
Sánchez-Fauquier, A .
CLINICAL MICROBIOLOGY AND INFECTION, 2003, 9 (04) :247-262
[40]  
Workowski KA, 2015, MMWR RECOMM REP, V64, P1