Efficacy of a recombinant M-like protein, SimA as a subunit vaccine candidate against Streptococcus parauberis infection in olive flounder, Paralichthys olivaceus

被引:6
作者
Kim, Tae-Ho [1 ]
Hwang, Seong Don [2 ]
Kim, Soo-Jin [1 ]
Kim, Myoung-Sug [1 ]
Choi, Hye-Sung [1 ,3 ]
Han, Hyun-Ja [1 ]
机构
[1] Natl Inst Fisheries Sci, Pathol Div, Busan 46083, South Korea
[2] Korea Maritime & Ocean Univ, Div Convergence Marine Sci, 727 Taejong ro, Busan 49112, South Korea
[3] 216 Gijanghaen ro, Busan 46083, South Korea
关键词
Streptococcus parauberis; Streptococcosis; Subunit vaccine; Recombinant protein; Aluminum hydroxide; M-like protein; Fibrinogen-binding protein; Olive flounder; Paralichthys olivaceus; EDWARDSIELLA-TARDA; INNATE IMMUNITY; CLASS-I; INIAE; ANTIGEN; ALPHA; EXPRESSION; RESISTANCE; CLONING; SINGLE;
D O I
10.1016/j.fsi.2022.10.009
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Streptococcus parauberis, a gram-positive cocci, causes bacterial disease in farmed fish. The recent increase in S. parauberis infection in aquatic farms in South Korea has justified the importance of vaccine development for the prevention of this disease. In this study, we evaluated the effect of subunit vaccines prepared from recom-binant M-like protein (SimA) and fibrinogen-binding protein (FBP) candidates with an aluminum hydroxide adjuvant against S. parauberis infection in olive flounder Paralichthys olivaceus. For the in vivo experiment, fish (average length, 7.18 cm; average weight, 3.5 g) were injected intraperitoneally with: phosphate buffer saline (PBS, group 1), PBS/aluminum hydroxide (group 2), FBP/aluminum hydroxide (group 3), SimA/aluminum hydroxide (group 4), and SimA/FBP/aluminum hydroxide (group 5). After 3 weeks, the fish in each group were boosted using PBS (group 1 and 2), FBP (group 3), SimA (group 4), and SimA/FBP (group 5) without adjuvant. We found that the relative percent survival of fish after S. parauberis exposure in group 2, 3, 4, and 5 was 6.25%, 18.75%, 50%, and 12.5%, respectively, whereas the mortality in groups 1 was 80%, respectively. We performed Western blot, ELISA, and quantitative real time RT-PCR (qRT-PCR) after vaccination to investigate the further efficacy of the vaccine. Western blot and ELISA of vaccinated fish serum confirmed the production of specific antibodies against SimA and FBP. Furthermore, results of qRT-PCR showed that recombinant protein SimA induced a remarkably specific-antibody response compared with that in FBP or control and increased the expression of various immune response-related genes including interleukin-8 (IL-8), toll-like receptor 2 (TLR2), tumor necrosis factor-alpha (TNF-alpha), CD4-1, and MHC II. Thus, these results indicate that SimA is a potent vaccine candidate for protection against S. parauberis infection.
引用
收藏
页码:1092 / 1100
页数:9
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