MiR-34c-3p targets Notch2 to inhibit cell invasion and epithelial-mesenchymal transition in nasopharyngeal carcinoma

被引：1

作者：

Jiang, Chengchuan ^{[1
]}

Zhou, Xiangqi ^{[2
]}

Zhu, Yuan ^{[3
]}

Mao, Yini ^{[1
]}

Wang, Ling ^{[4
]}

Kuang, Yuqing ^{[5
]}

Su, Ju ^{[5
]}

Huang, Weiguo ^{[6
]}

Tang, Sanyuan ^{[1
,2
]}

机构：

[1] Brain Hosp Hunan Prov, Oncol Dept, Changsha, Hunan, Peoples R China

[2] Univ South China, Oncol Dept, Affiliated Nanhua Hosp, Hengyang, Peoples R China

[3] Peoples Hosp Changshou Chongqing, Oncol Dept, Chongqing, Peoples R China

[4] Yi Chang Cent Peoples Hosp, Yi Chang, Hubei, Peoples R China

[5] Xiangxi Autonomous Prefecture Peoples Hosp, Jishou, Hunan, Peoples R China

[6] Univ South China, Canc Res Inst, Hengyang Med Coll, Hengyang, Hunan, Peoples R China

来源：

FOOD SCIENCE AND TECHNOLOGY | 2022年 / 42卷

关键词：

nasopharyngeal carcinoma; miR-34c-3p; Notch2; epithelial-mesenchymal transition; COLORECTAL-CANCER; DOWN-REGULATION; SUPPRESSES; PROLIFERATION; GROWTH; PROGRESSION; METASTASIS; APOPTOSIS;

D O I：

10.1590/fst.67421

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study was to investigate role of miR-34c-3p/Notch2-EMT signal axis in Nasopharyngeal Carcinoma (NPC). Ten samples of NPC tissues and chronic inflammatory tissues of nasopharynx (CITN) were collected. 133 differentially expressed miRNAs, including 31 down-regulated and 102 up-regulated were identified in NPC tissues. MiR-34c-3p was down-regulated and Notch2 was up-regulated in NPC tissues compared with CITN tissues. MiR-34c-3p was overexpressed in 6-10B cells after transfected with miR-34c-3p mimic, while reduced in 5-8F cells after transfected with miR-34c-3p inhibitor. Notch2 was confirmed as a target gene of miR-34c-3p. miR-34c-3p overexpression in 6-10B cells suppressed, while knockdown in 5-8F cells promoted cell invasion ability. In molecular level, the expression of E-cadherin was increased, while N-cadherin was decreased after miR-34c-3p overexpression in 6-10B cells. Knockdown of miR-34c-3p obviously decreased E-cadherin, but increased N-cadherin expression in 5-8F cells. Targeting Notch2 by miR-34c-3p might be a promising target for NPS treatment.

引用

页数：10

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