MiR-34c-3p targets Notch2 to inhibit cell invasion and epithelial-mesenchymal transition in nasopharyngeal carcinoma

被引:1
作者
Jiang, Chengchuan [1 ]
Zhou, Xiangqi [2 ]
Zhu, Yuan [3 ]
Mao, Yini [1 ]
Wang, Ling [4 ]
Kuang, Yuqing [5 ]
Su, Ju [5 ]
Huang, Weiguo [6 ]
Tang, Sanyuan [1 ,2 ]
机构
[1] Brain Hosp Hunan Prov, Oncol Dept, Changsha, Hunan, Peoples R China
[2] Univ South China, Oncol Dept, Affiliated Nanhua Hosp, Hengyang, Peoples R China
[3] Peoples Hosp Changshou Chongqing, Oncol Dept, Chongqing, Peoples R China
[4] Yi Chang Cent Peoples Hosp, Yi Chang, Hubei, Peoples R China
[5] Xiangxi Autonomous Prefecture Peoples Hosp, Jishou, Hunan, Peoples R China
[6] Univ South China, Canc Res Inst, Hengyang Med Coll, Hengyang, Hunan, Peoples R China
来源
FOOD SCIENCE AND TECHNOLOGY | 2022年 / 42卷
关键词
nasopharyngeal carcinoma; miR-34c-3p; Notch2; epithelial-mesenchymal transition; COLORECTAL-CANCER; DOWN-REGULATION; SUPPRESSES; PROLIFERATION; GROWTH; PROGRESSION; METASTASIS; APOPTOSIS;
D O I
10.1590/fst.67421
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
This study was to investigate role of miR-34c-3p/Notch2-EMT signal axis in Nasopharyngeal Carcinoma (NPC). Ten samples of NPC tissues and chronic inflammatory tissues of nasopharynx (CITN) were collected. 133 differentially expressed miRNAs, including 31 down-regulated and 102 up-regulated were identified in NPC tissues. MiR-34c-3p was down-regulated and Notch2 was up-regulated in NPC tissues compared with CITN tissues. MiR-34c-3p was overexpressed in 6-10B cells after transfected with miR-34c-3p mimic, while reduced in 5-8F cells after transfected with miR-34c-3p inhibitor. Notch2 was confirmed as a target gene of miR-34c-3p. miR-34c-3p overexpression in 6-10B cells suppressed, while knockdown in 5-8F cells promoted cell invasion ability. In molecular level, the expression of E-cadherin was increased, while N-cadherin was decreased after miR-34c-3p overexpression in 6-10B cells. Knockdown of miR-34c-3p obviously decreased E-cadherin, but increased N-cadherin expression in 5-8F cells. Targeting Notch2 by miR-34c-3p might be a promising target for NPS treatment.
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页数:10
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