MiR-34c-3p targets Notch2 to inhibit cell invasion and epithelial-mesenchymal transition in nasopharyngeal carcinoma

被引：1

作者：

Jiang, Chengchuan ^{[1
]}

Zhou, Xiangqi ^{[2
]}

Zhu, Yuan ^{[3
]}

Mao, Yini ^{[1
]}

Wang, Ling ^{[4
]}

Kuang, Yuqing ^{[5
]}

Su, Ju ^{[5
]}

Huang, Weiguo ^{[6
]}

Tang, Sanyuan ^{[1
,2
]}

机构：

[1] Brain Hosp Hunan Prov, Oncol Dept, Changsha, Hunan, Peoples R China

[2] Univ South China, Oncol Dept, Affiliated Nanhua Hosp, Hengyang, Peoples R China

[3] Peoples Hosp Changshou Chongqing, Oncol Dept, Chongqing, Peoples R China

[4] Yi Chang Cent Peoples Hosp, Yi Chang, Hubei, Peoples R China

[5] Xiangxi Autonomous Prefecture Peoples Hosp, Jishou, Hunan, Peoples R China

[6] Univ South China, Canc Res Inst, Hengyang Med Coll, Hengyang, Hunan, Peoples R China

来源：

FOOD SCIENCE AND TECHNOLOGY | 2022年 / 42卷

关键词：

nasopharyngeal carcinoma; miR-34c-3p; Notch2; epithelial-mesenchymal transition; COLORECTAL-CANCER; DOWN-REGULATION; SUPPRESSES; PROLIFERATION; GROWTH; PROGRESSION; METASTASIS; APOPTOSIS;

D O I：

10.1590/fst.67421

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study was to investigate role of miR-34c-3p/Notch2-EMT signal axis in Nasopharyngeal Carcinoma (NPC). Ten samples of NPC tissues and chronic inflammatory tissues of nasopharynx (CITN) were collected. 133 differentially expressed miRNAs, including 31 down-regulated and 102 up-regulated were identified in NPC tissues. MiR-34c-3p was down-regulated and Notch2 was up-regulated in NPC tissues compared with CITN tissues. MiR-34c-3p was overexpressed in 6-10B cells after transfected with miR-34c-3p mimic, while reduced in 5-8F cells after transfected with miR-34c-3p inhibitor. Notch2 was confirmed as a target gene of miR-34c-3p. miR-34c-3p overexpression in 6-10B cells suppressed, while knockdown in 5-8F cells promoted cell invasion ability. In molecular level, the expression of E-cadherin was increased, while N-cadherin was decreased after miR-34c-3p overexpression in 6-10B cells. Knockdown of miR-34c-3p obviously decreased E-cadherin, but increased N-cadherin expression in 5-8F cells. Targeting Notch2 by miR-34c-3p might be a promising target for NPS treatment.

引用

页数：10

共 50 条

[11] MiR-101-3p targets the PI3K-AKT signaling pathway via Birc5 to inhibit invasion, proliferation, and epithelial-mesenchymal transition in hepatocellular carcinoma [J].

Zhu, Wenyuan ;

Ni, Qingqiang ;

Wang, Zhengjian ;

Zhang, Ruxuan ;

Liu, Fangfeng ;

Chang, Hong .

CLINICAL AND EXPERIMENTAL MEDICINE, 2025, 25 (01)

[12] miR-184 Inhibits Tumor Invasion, Migration and Metastasis in Nasopharyngeal Carcinoma by Targeting Notch2 [J].

Zhu, Hong-Ming ;

Jiang, Xue-Song ;

Li, Hui-Zi ;

Qian, Lu-Xi ;

Du, Ming-Yu ;

Lu, Zhi-Wei ;

Wu, Jing ;

Tian, Xiao-Kang ;

Fei, Qian ;

He, Xia ;

Yin, Li .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 49 (04) :1564-1576

[13] MMP14 regulates cell migration and invasion through epithelial-mesenchymal transition in nasopharyngeal carcinoma [J].

Yan, Tinghua ;

Lin, Zhonghao ;

Jiang, Jinhua ;

Lu, Suiwan ;

Chen, Miaoan ;

Que, Huaxing ;

He, Xiangsheng ;

Que, Ganbo ;

Mao, Jianfeng ;

Xiao, Jinan ;

Zheng, Qingwei .

AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2015, 7 (05) :950-958

[14] Regulation of cancerous progression and epithelial-mesenchymal transition by miR-34c-3p via modulation of MAP3K2 signaling in triple-negative breast cancer cells [J].

Wu, Jiang ;

Li, Wei-zhi ;

Huang, Mei-ling ;

Wei, Hong-liang ;

Wang, Ting ;

Fan, Jing ;

Li, Nan-lin ;

Ling, Rui .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 483 (01) :10-16

[15] miR-223-3p targets FBXW7 to promote epithelial-mesenchymal transition and metastasis in breast cancer [J].

Wang, Yuli ;

Shi, Shuang ;

Wang, Yunshan ;

Zhang, Xuhua ;

Liu, Xiaoyan ;

Li, Juan ;

Li, Peilong ;

Du, Lutao ;

Wang, Chuanxin .

THORACIC CANCER, 2022, 13 (03) :474-482

[16] miR-574-3p Regulates Smad/Snail Signaling to Promote Epithelial-Mesenchymal Transition in Nasopharyngeal Carcinoma Cells [J].

Ai, Ping ;

Qu, Wei ;

Peng, Xianbing ;

Zeng, Yi .

BIOCHEMICAL GENETICS, 2025,

[17] Let-7a inhibits migration, invasion and epithelial-mesenchymal transition by targeting HMGA2 in nasopharyngeal carcinoma [J].

Wu, Aibing ;

Wu, Kunpeng ;

Li, Jinmei ;

Mo, Yanli ;

Lin, Yanming ;

Wang, Yuzhou ;

Shen, Xiang ;

Li, Shujun ;

Li, Lixia ;

Yang, Zhixiong .

JOURNAL OF TRANSLATIONAL MEDICINE, 2015, 13

[18] Tumor-educated platelet miR-34c-3p and miR-18a-5p as potential liquid biopsy biomarkers for nasopharyngeal carcinoma diagnosis [J].

Wang, Hui ;

Wei, Xiuqi ;

Wu, Bian ;

Su, Jingyu ;

Tan, Wenbin ;

Yang, Kunyu .

CANCER MANAGEMENT AND RESEARCH, 2019, 11 :3351-3360

[19] hsa-miR-24 suppresses metastasis in nasopharyngeal carcinoma by regulating the c-Myc/epithelial-mesenchymal transition axis [J].

Su, Bojin ;

Xu, Tao ;

Bruce, Jeff P. ;

Yip, Kenneth W. ;

Zhang, Ning ;

Huang, Zeli ;

Zhang, Guoyi ;

Liu, Fei-Fei ;

Liang, Jiyong ;

Yang, Huiling ;

Claret, Francois X. .

ONCOLOGY REPORTS, 2018, 40 (05) :2536-2546

[20] Differential effects of miR-34c-3p and miR-34c-5p on the proliferation, apoptosis and invasion of glioma cells [J].

Wu, Zhendong ;

Wu, Yupeng ;

Tian, Ye ;

Sun, Xiaofeng ;

Liu, Jixiang ;

Ren, Hongbo ;

Liang, Chaohui ;

Song, Ligang ;

Hu, Hongchao ;

Wang, Liqun ;

Jiao, Baohua .

ONCOLOGY LETTERS, 2013, 6 (05) :1447-1452

← 1 2 3 4 5 →