Comparison of the growth kinetics of neuropathogenic and nonneuropathogenic equid herpesvirus type 1 (EHV-1) strains in cultured murine neuronal cells and the relevance of the D/N752 coding change in DNA polymerase gene (ORF30)

被引：19

作者：

Yamada, Souichi ^{[2
]}

Matsumura, Tomio ^{[3
]}

Tsujimura, Koji ^{[3
]}

Yamaguchi, Tsuyoshi ^{[4
]}

Ohya, Kenji ^{[1
]}

Fukushi, Hideto ^{[1
,2
]}

机构：

[1] Gifu Univ, Fac Appl Biol Sci, Lab Vet Microbiol, Gifu 5011193, Japan

[2] Gifu Univ, United Grad Sch Vet Sci, Dept Appl Vet Sci, Gifu 5011193, Japan

[3] Japan Racing Assoc, Equine Res Inst, Epizoot Res Ctr, Div Mol Biol, Shimotsuke, Tochigi 3290412, Japan

[4] Tottori Univ, Fac Agr, Avian Zoonosis Res Ctr, Tottori 6808550, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2008年 / 70卷 / 05期

关键词：

DNA polymerase; EHV-1; neuron;

D O I：

10.1292/jvms.70.505

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We compared the growth kinetics of neuropathogenic and nonneuropathogenic equine herpesvirus type 1 (EHV-1) strains in mouse cerebral cortex cells and investigated the relevance of the D/N amino acid change at position 752 of ORF30 in Japanese isolates. Neuropathogenic electropherotype P strains 01c1 and 89c25 exhibited similar growth kinetics to nonneuropathogenic P strain 90c16 in cultured neurons; however, the growth ability of type B strain 97c7 was lower than those of the other strains tested. The amino acid encoded at 752 of ORF30 in 01c1 was asparagic acid; asparagine was encoded in the other EHV-1 strains isolated from Japanese horses. The D/N-752 difference in ORF30 may not be related to replication ability in neurons.

引用

页码：505 / 511

页数：7

共 24 条

[1]

Abramoff MD., 2004, Biophot. Int., V11, P36

[2] Quantification by real-time PCR of the magnitude and duration of leucocyte-associated viraemia in horses infected with neuropathogenic vs. non-neuropathogenic strains of EHV-1 [J].

Allen, G. P. ;

Breathnach, C. C. .

EQUINE VETERINARY JOURNAL, 2006, 38 (03) :252-257

[3]

ALLEN GP, 1983, AM J VET RES, V44, P263

[4] Pathogenesis of equine herpesvirus-associated neurological disease: a revised explanation [J].

Borchers, K. ;

Thein, P. ;

Sterner-Kock, A. .

EQUINE VETERINARY JOURNAL, 2006, 38 (03) :283-287

[5] Initial characterization of 17 viruses harboring mutant forms of the immediate-early gene of equine herpesvirus 1 [J].

Buczynski, KA ;

Kim, SK ;

O'Callaghan, DJ .

VIRUS GENES, 2005, 31 (02) :229-239

[6] Meningoencephalitis in mice infected with an equine herpesvirus 1 strain KyA recombinant expressing glycoprotein I and glycoprotein D [J].

Frampton, AR ;

Smith, PM ;

Zhang, YF ;

Grafton, WD ;

Matsumura, T ;

Osterrieder, N ;

O'Callaghan, DJ .

VIRUS GENES, 2004, 29 (01) :9-17

[7] Gazelle herpesvirus 1: A new neurotropic herpesvirus immunologically related to equine herpesvirus 1 [J].

Fukushi, H ;

Tomita, T ;

Taniguchi, A ;

Ochiai, Y ;

Kirisawa, R ;

Matsumura, T ;

Yanai, T ;

Masegi, T ;

Yamaguchi, T ;

Hirai, K .

VIROLOGY, 1997, 227 (01) :34-44

[8] A hamster model of equine herpesvirus 9 induced encephalitis [J].

Fukushi, H ;

Taniguchi, A ;

Yasuda, K ;

Yanai, T ;

Masegi, T ;

Yamaguchi, T ;

Hirai, K .

JOURNAL OF NEUROVIROLOGY, 2000, 6 (04) :314-319

[9] THE PATHOGENICITY OF AB4P, THE SEQUENCED STRAIN OF EQUINE HERPESVIRUS-1, IN SPECIFIC PATHOGEN-FREE FOALS [J].

GIBSON, JS ;

SLATER, JD ;

FIELD, HJ .

VIROLOGY, 1992, 189 (01) :317-319

[10] Equine herpesvirus-1-induced encephalomyelitis in mice: a comparative study of neuroadapted virus and its parental strain [J].

Hasebe, R ;

Kimura, T ;

Sato, E ;

Okazaki, K ;

Ochiai, K ;

Wada, R ;

Umemura, T .

JOURNAL OF COMPARATIVE PATHOLOGY, 2002, 127 (2-3) :118-125

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