Pseudomonas bauzanensis sp nov., isolated from soil

A Gram-negative, aerobic, motile rod, designated BZ93(T), was isolated from soil from an industrial site. The strain grew at 5-30 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ93(T) was related to members of the genus Pseudomonas and was related most closely to Pseudomonas xiamenensis C10-2(T) (97.8% 16S rRNA gene sequence similarity) and Pseudomonas pertucinogena IFO 14163(T) (97.4%). The predominant cellular fatty acids of strain BZ93(T) were C-18:1 omega 7c (54.8%), summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH; 10.3%), C-16:0 (9.9%) and C-17:0 cyclo (7.4%). The major quinone was ubiquinone 9. The major phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. The genomic DNA G+C content was 61.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Pseudomonas bauzanensis sp. nov., is proposed. The type strain is BZ93(T) (=DSM 22558(T) =CGMCC 1.9095(T)=LMG 26048(T)).