Dental caries imaging using hyperspectral stimulated Raman scattering microscopy

被引:0
|
作者
Wang Zi [1 ]
Zheng Wei [1 ]
Jian Lin [1 ]
Huang Zhiwei [1 ]
机构
[1] Natl Univ Singapore, Fac Engn, Dept Biomed Engn, Opt Bioimaging Lab, Singapore 117576, Singapore
来源
BIOMEDICAL VIBRATIONAL SPECTROSCOPY 2016: ADVANCES IN RESEARCH AND INDUSTRY | 2016年 / 9704卷
关键词
stimulated Raman scattering; spectroscopic imaging; tooth;
D O I
10.1117/12.2213793
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We report the development of a polarization-resolved hyperspectral stimulated Raman scattering (SRS) imaging technique based on a picosecond (ps) laser-pumped optical parametric oscillator system for label-free imaging of dental caries. In our imaging system, hyperspectral SRS images (512x512 pixels) in both fingerprint region (800-1800 cm(-1)) and high-wavenumber region (2800-3600 cm(-1)) are acquired in minutes by scanning the wavelength of OPO output, which is a thousand times faster than conventional confocal micro Raman imaging. SRS spectra variations from normal enamel to caries obtained from the hyperspectral SRS images show the loss of phosphate and carbonate in the carious region. While polarization-resolved SRS images at 959 cm(-1) demonstrate that the caries has higher depolarization ratio. Our results demonstrate that the polarization resolved-hyperspectral SRS imaging technique developed allows for rapid identification of the biochemical and structural changes of dental caries.
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页数:5
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