Reduced mitochondrial DNA transcription in senescent rat heart

被引：32
作者：
Andreu, AL
Arbos, MA
Perez-Martos, A
Lopez-Perez, MJ
Asin, J
Lopez, N
Montoya, J
Schwartz, S
机构：
[1] Hosp Gen Valle Hebron, Ctr Invest Bioquim & Biol Mol, E-08035 Barcelona, Spain
[2] Univ Zaragoza, Dept Bioquim & Biol Mol & Celular, E-50013 Zaragoza, Spain
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 252卷 / 03期
关键词：
mitochondrial DNA; aging; transcription in vitro; OXPHOS system; oxidative damage; gene expression;
D O I：
10.1006/bbrc.1998.9703
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We studied the effect of senescence on mitochondrial DNA (mtDNA) transcription with an in organello system using intact isolated rat heart mitochondria. A comparison of the electrophoretic patterns of mtDNA transcription products in young, adult and senescent rats showed an age-related reduction in newly-synthesized mitochondrial RNAs that reflects a decrease in the synthesis rate. These results correlate with the enzyme activities of the oxidative phosphorylation complexes I and IV, that are partially encoded by the mitochondrial genome. In addition, an age-related increase in the protein carbonyl content of the mitochondrial membranes was observed in senescent mitochondria suggesting an accumulation of mitochondrial oxidative damage. This reduction in the mtDNA transcriptional rate in the heart of senescent animals suggests that this could be one of the molecular bases underlying senescence of the myocardium. (C) 1998 Academic Press.
引用
收藏
页码：577 / 581
页数：5
相关论文
共 37 条
[1]  
AMBROSIO G, 1993, J BIOL CHEM, V268, P18532
[2]  
ATTARDI G, 1983, METHODS ENZYMOL, V97
[3]  
ATTARDI G, 1985, INT REV CYTOL, V93, P93145
[4]   AN EVALUATION OF THE MEASUREMENT OF THE ACTIVITIES OF COMPLEXES I-IV IN THE RESPIRATORY-CHAIN OF HUMAN SKELETAL-MUSCLE MITOCHONDRIA [J].
BIRCHMACHIN, MA ;
BRIGGS, HL ;
SABORIDO, AA ;
BINDOFF, LA ;
TURNBULL, DM .
BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY, 1994, 51 (01) :35-42
[5]  
Caldarera C M, 1991, Cardiologia, V36, P231
[6]  
CALLEJA M, 1993, J BIOL CHEM, V268, P18891
[7]   MITOCHONDRIAL ACTIVITIES OF RAT-HEART DURING AGING [J].
CASTELLUCCIO, C ;
BARACCA, A ;
FATO, R ;
PALLOTTI, F ;
MARANESI, M ;
BARZANTI, V ;
GORINI, A ;
VILLA, RF ;
CASTELLI, GP ;
MARCHETTI, M ;
LENAZ, G .
MECHANISMS OF AGEING AND DEVELOPMENT, 1994, 76 (2-3) :73-88
[8]  
Enriquez J A, 1996, Methods Enzymol, V264, P50, DOI 10.1016/S0076-6879(96)64008-7
[9]   SATURATION OF THE PROCESSING OF NEWLY SYNTHESIZED RIBOSOMAL-RNA IN ISOLATED BRAIN MITOCHONDRIA [J].
ENRIQUEZ, JA ;
LOPEZPEREZ, MJ ;
MONTOYA, J .
FEBS LETTERS, 1991, 280 (01) :32-36
[10]   SPECIFIC INCREASE OF A MITOCHONDRIAL RNA TRANSCRIPT IN CHRONIC ETHANOL-FED RATS [J].
ENRIQUEZ, JA ;
PEREZMARTOS, A ;
FERNANDEZSILVA, P ;
LOPEZPEREZ, MJ ;
MONTOYA, J .
FEBS LETTERS, 1992, 304 (2-3) :285-288
1234