Crystallization and preliminary X-ray diffraction analysis of the small laccase from Streptomyces coelicolor

被引：14

作者：

Skalova, Tereza ^{[1
]}

Dohnalek, Jan ^{[1
,2
]}

Ostergaard, Lars Henrik ^{[3
]}

Ostergaard, Peter Rahbek ^{[3
]}

Kolenko, Petr ^{[1
]}

Duskova, Jarmila ^{[1
]}

Hasek, Jindrich ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Macromol Chem, CZ-16206 Prague, Czech Republic

[2] Acad Sci Czech Republ, Inst Phys, CZ-16253 Prague, Czech Republic

[3] Novozymes A S, DK-2880 Bagsvaerd, Denmark

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷

关键词：

Laccases; Multicopper blue proteins; Oxidoreductases;

D O I：

10.1107/S1744309107060721

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The small bacterial laccase from the actinobacterium Streptomyces coelicolor which lacks the second of the three domains of the laccases structurally characterized to date was crystallized. This multi-copper phenol oxidase crystallizes in a primitive tetragonal lattice, with unit-cell parameters a = b = 179.8, c = 175.3 angstrom. The crystals belong to either space group P4(1)2(1)2 or P4(3)2(1)2. The self-rotation function shows the presence of a noncrystallographic threefold axis in the structure. Phases will be determined from the anomalous signal of the natively present copper ions.

引用

页码：1077 / 1079

页数：3

共 18 条

[1] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[2] HIGH-LEVEL EXPRESSION OF RECOMBINANT GENES IN ASPERGILLUS-ORYZAE [J].

CHRISTENSEN, T ;

WOELDIKE, H ;

BOEL, E ;

MORTENSEN, SB ;

HJORTSHOEJ, K ;

THIM, L ;

HANSEN, MT .

BIO-TECHNOLOGY, 1988, 6 (12) :1419-1422

[3] Industrial and biotechnological applications of laccases: A review [J].

Couto, Susana Rodriguez ;

Herrera, Jose Luis Toca .

BIOTECHNOLOGY ADVANCES, 2006, 24 (05) :500-513

[4]

CUDNEY B, 1994, ACTA CRYSTALLOGR D, V50, P414, DOI 10.1107/S0907444994002660

[5] Structure of the laccase from Coprinus cinereus at 1.68 Å resolution:: evidence for different 'type 2 Cu-depleted′ isoforms [J].

Ducros, V ;

Brzozowski, AM ;

Wilson, KS ;

Ostergaard, P ;

Schneider, P ;

Svendson, A ;

Davies, GJ .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 :333-336

[6] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411

[7]

LESLIE AGW, 1992, INT CCP4 ESF EACBM N, V26

[8] Characterization of SLAC:: A small laccase from Streptomyces coelicolor with unprecedented activity [J].

Machczynski, MC ;

Vijgenboom, E ;

Samyn, B ;

Canters, GW .

PROTEIN SCIENCE, 2004, 13 (09) :2388-2397

[9] ENZYMOLOGY OF OXYGEN [J].

MALMSTROM, BG .

ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :21-59

[10] Laccase: new functions for an old enzyme [J].

Mayer, AM ;

Staples, RC .

PHYTOCHEMISTRY, 2002, 60 (06) :551-565

← 1 2 →