Introduction: From its androcentric vision and through the prevailing patriarchal society in this field over the years, sport has constrained, not infrequently, the action and personal development of those people who are not governed by socially established rules. In this sense, this situation affects not only the group of homosexuals, bisexuals, transsexuals, and intersexuals (LGBTI), but all those individuals who do not follow the pattern established by heteronormativity. Although it is true that there have been notable advances in this regard in our society, evolving towards a climate of greater tolerance, the presence of numerous situations dominated by discrimination and violence towards those minorities who question heterosexual norms is still present. Therefore, it is necessary to make use of the healthy educational, social, cultural, and psychological functions that physical-sports activities can play towards people. Objectives: The main objective of this study was to evaluate the attitude of Secondary Education students towards sexual diversity in the sports environment. Methods: The study involved 87 students ranging in age from 15 to 17 years old. The Attitudes towards Sexual Diversity in Sport Scale was used to determine students' attitudes towards sexual diversity. Results: In general, positive attitudes towards sexual diversity in sport were found, boys scoring more negatively than girls, mainly towards transgender individuals. Based on these results, it is evident that some of the students have negative attitudes towards sexual diversity, hence, proposing educational action lines that work against this situation is considered necessary.