UPLC-PDA-MS evaluation of bioactive compounds from leaves of Ilex paraguariensis with different growth conditions, treatments and ageing

被引：84

作者：

Dartora, Nessana ^{[1
]}

de Souza, Lauro M. ^{[1
]}

Santana-Filho, Arquimedes P. ^{[1
]}

Iacomini, Marcello ^{[1
]}

Valduga, Alice T. ^{[2
]}

Gorin, Philip A. J. ^{[1
]}

Sassaki, Guilherme L. ^{[1
]}

机构：

[1] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana, Brazil

[2] Univ Reg Integrada Alto Uruguai & Missoes, Dept Ciencias Biol, Erechim, RS, Brazil

来源：

FOOD CHEMISTRY | 2011年 / 129卷 / 04期

关键词：

Ilex paraguariensis; UPLC-MS analysis; Phenols; Xanthines; Carbohydrates; FERMENTATION TIME; MASS-SPECTROMETRY; ANTIOXIDANTS; QUALITY; TEA; CONSTITUENTS; CHLOROPHYLL;

D O I：

10.1016/j.foodchem.2011.05.112

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Teas from Mate have been widely consumed for centuries in Brazil, Argentina, Paraguay, and Uruguay. We now determine how age, growth conditions and post-harvesting processes of leaves from Ilex paraguariensis affect the concentration of bioactive compounds and their antioxidant capacity. Phenolics, xanthines, and carbohydrates were identified and quantified by electrospray ionisation mass spectrometry (ESI-MS) and ultra performance liquid chromatography (UPLC), which dramatically reduces the time for each analysis (<3 min). On average, sun-exposed (monoculture) leaf extracts exhibited higher levels of bioactive compounds as compared to shaded (forest grown) ones. PCA (principal component analysis) analysis of all the samples indicated that those obtained after blanching and drying contained more phenolics and a smaller concentration of xanthine than those in natura. The oxidised leaves had lower concentrations of phenolics, and consequently a decline in antioxidant activity. No differences were found based on the leaf age. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1453 / 1461

页数：9

共 34 条

[1] NATURAL CONSTITUENTS OF ILEX SPECIES
ALIKARIDIS, F
[J]. JOURNAL OF ETHNOPHARMACOLOGY, 1987, 20 (02) : 121 - 144
[2] [Anonymous], 2002, NATURE, DOI DOI 10.1038/1811199A0
[3] Physiological postharvest responses of Common Saint-John's wort herbs (Hypericum perforatum L.)
Böttcher, H
Günther, I
Kabelitz, L
[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY, 2003, 29 (03) : 343 - 351
[4] LC/MS characterization of phenolic constituents of mate (Ilex paraguariensis, St. Hil.) and its antioxidant activity compared to commonly consumed beverages
Bravo, Laura
Goya, Luis
Lecumberri, Elena
[J]. FOOD RESEARCH INTERNATIONAL, 2007, 40 (03) : 393 - 405
[5] Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents)
Calixto, JB
[J]. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2000, 33 (02) : 179 - 189
[6] Carducci CN, 2000, J AOAC INT, V83, P1167
[7] Carini M, 1998, RAPID COMMUN MASS SP, V12, P1813, DOI 10.1002/(SICI)1097-0231(19981130)12:22<1813::AID-RCM379>3.0.CO
[8] 2-#
[9] Croce Dorli Mário Da, 2002, Ciênc. Florest., V12, P107, DOI 10.5902/198050981685
[10] Analysis of flavonol glycoside isomers from leaves of Maytenus ilicifolia by offline and online high performance liquid chromatography-electrospray mass spectrometry
de Souza, Lauro M.
Cipriani, Thales R.
Serrato, Rodrigo V.
da Costa, Denise E.
Iacomini, Marcello
Gorin, Philip A. J.
Sassaki, Guilherme L.
[J]. JOURNAL OF CHROMATOGRAPHY A, 2008, 1207 (1-2) : 101 - 109

← 1 2 3 4 →