Regulation of macrophage inflammatory protein-2 gene expression in response to 2,4-dinitrofluorobenzene in RAW 264.7 cells

被引：12

作者：

Kim, Dongbum ^{[1
]}

Kim, Jinho ^{[2
]}

Kwon, Sanghoon ^{[3
]}

Kim, Youy-Jin ^{[3
]}

Lee, Soohyoung ^{[3
]}

Lee, Younghee ^{[4
]}

Seo, Jae-Nam ^{[5
]}

Park, Cheung-Seog ^{[6
]}

Park, Kui Lea ^{[2
]}

Kwon, Hyung-Joo ^{[1
,3
]}

机构：

[1] Hallym Univ, Ctr Med Sci Res, Gangwon, Chuncheon, South Korea

[2] Natl Inst Toxicol Res, Immunotoxicol Team, Seoul, South Korea

[3] Hallym Univ, Dept Microbiol, Chunchon, Gangwon, South Korea

[4] Chungbuk Natl Univ, Coll Nat Sci, Dept Biochem, Chungbuk, South Korea

[5] Hallym Univ, Coll Med, Dept Pathol, Chunchon, Gangwon, South Korea

[6] Kyung Hee Univ, Coll Med, Dept Microbiol, Seoul, South Korea

来源：

BMB REPORTS | 2008年 / 41卷 / 04期

关键词：

AP-1; DNFB; macrophages; MIP-2;

D O I：

10.5483/BMBRep.2008.41.4.316

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several skin sensitizers, like 2,4-dinitrofluorobenzene (DNFB), are known to provoke contact hypersensitivity responses after topical application. Here, we show that DNFB can upregulate macrophage inflammatory protein-2 (MIP-2) expression in RAW 264.7 cells via a mechanism that is largely dependent on mitogen-activated protein kinase (MAPK) signaling pathways. ELISA-based transcription factor activation assays and chromatin immunoprecipitation assays revealed that functional interaction between AP-1 and MIP-2 promoter element is necessary for MIP-2 gene expression by DNFB. Interestingly, topical application of DNFB to NC/Nga mice increased MIP-2 expression in dermis, suggesting that MIP-2 contributes to the leukocyte infiltration. associated with atopic dermatitis. These results provide additional insight of the mechanism of contact hypersensitivity induced by contact sensitizers.

引用

页码：316 / 321

页数：6

共 22 条

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