Identification of exocytotic membrane proteins in three rotifer species

被引：5

作者：

Alejandro Perez-Legaspi, Ignacio ^{[1
]}

Del Rosario Montoya-Garcia, Maria ^{[2
]}

Quintanar, J. Luis ^{[2
]}

Salinas, Eva ^{[3
]}

Rico-Martinez, Roberto ^{[1
]}

机构：

[1] Univ Autonoma Aguascalientes, Dept Quim, Aguascalientes 20100, Mexico

[2] Univ Autonoma Aguascalientes, Dept Fisiol & Farmacol, Aguascalientes 20100, Mexico

[3] Univ Autonoma Aguascalientes, Dept Microbiol, Aguascalientes 20100, Mexico

来源：

HYDROBIOLOGIA | 2008年 / 600卷 / 1期

关键词：

immunoblot; immunohistochemistry; exocytosis; SNAREs;

D O I：

10.1007/s10750-007-9184-0

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

We confirmed the presence and expression of exocytotic membrane proteins: Syntaxin-1, Syntaxin-4, SNAP-23, and SNAP-25 in the rotifers Brachionus calyciflorus, Lecane quadridentata, and Philodina roseola. These proteins were identified by immunohistochemistry and immunoblot analysis using antibodies against Syntaxin-1, Syntaxin-4, SNAP-23, and SNAP-25. The presence of these proteins were observed mainly in regions of the nervous, reproductive, and glandular systems of these rotifer species, which underlines the fundamental role of exocytotic docking and fusion membrane proteins in the vesicular release of secretory proteins. The immunoblot analysis confirmed the expression of Syntaxin-1, Syntaxin-4, and SNAP-23 in the three rotifer studied, but not of SNAP-25. This study contributes to rotifer biology by revealing the presence of conservative exocytotic machinery such as SNAREs proteins in the phylum Rotifera, including members of the Class Bdelloidea the highest taxa of metazoans where sexual reproduction does not exist.

引用

页码：147 / 154

页数：8

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