Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex

被引：128
作者：
Chen, S
Huang, X
Zhou, XF
Bai, LQ
He, J
Jeong, KJ
Lee, SY [1 ]
Deng, ZX
机构：
[1] Shanghai Jiao Tong Univ, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China
[3] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
[4] Huazhong Agr Univ, Sch Life Sci & Technol, Wuhan 430070, Peoples R China
来源：
CHEMISTRY & BIOLOGY | 2003年 / 10卷 / 11期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.chembiol.2003.10.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The complete gene cluster for biosynthesis of a polyene complex, FR-008, spans 137.2 kb of the genome of Streptomyces sp. FR-008 consisting of six genes for a modular PKS and 15 additional genes. The extensive similarity to the partially characterized candicidin gene cluster in Streptomyces griseus IMRU3570, especially for genes involved in mycosamine biosynthesis, prompted us to compare the compounds produced by Streptomyces sp. FR-008 and Streptomyces griseus IMRU3570, and we found that FR-008 and candicidin complex are identical. A model for biosynthesis of a set of four structurally related FR-008/candicidin compounds was proposed. Deletion of the putative regulatory genes abolished antibiotic production, while disruption of putative glycosyltransferase and GDP-ketosugar aminotransferase functionalities led to the productions of a set of nonmycosaminated aglycones and a novel polyene complex with attachment of altered sugar moiety, respectively.
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页码：1065 / 1076
页数：12
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