Efficiency of pyrimidine dimer formation in Escherichia coli across UV wavelengths

被引：24

作者：

Eischeid, A. C. ^{[1
]}

Linden, K. G. ^{[1
]}

机构：

[1] Duke Univ, Dept Civil & Environm Engn, Durham, NC 27708 USA

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2007年 / 103卷 / 05期

关键词：

bacteria; cyclobutane pyrimidine dimer; enzyme sensitive site assay; Escherichia coli; low-pressure ultraviolet; medium-pressure ultraviolet; ultraviolet disinfection;

D O I：

10.1111/j.1365-2672.2007.03424.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: Inactivation of Escherichia coli as a function of ultraviolet (UV) wavelength was investigated by using the endonuclease-sensitive site (ESS) assay to quantify pyrimidine dimer formation. Methods and Results: Ultraviolet dose-response curves were determined based on both log reduction in colony-forming units (CFU) and endonuclease-sensitive sites per kb DNA (ESS/kb) for monochromatic 254-nm low-pressure (LP) UV, polychromatic medium-pressure (MP) UV, 228 and 289-nm UV irradiation. UV irradiation from LP and MP UV sources were approx. equal in both CFU reduction and pyrimidine dimer formation at all UV doses studied; 228-nm irradiation was less effective than LP or MP, and 289-nm irradiation was the least effective in both CFU reduction and pyrimidine dimer formation. These results are in qualitative agreement with the absorption spectrum of pyrimidine bases in DNA. Results indicated an approx. linear relationship between ESS/kb and log CFU reduction. Conclusions: Formation of pyrimidine dimers in genomic DNA is primarily responsible for UV inactivation of E. coli. Significance and Impact of the Study: This work contributed to fundamental understanding of UV disinfection and aids in UV reactor design.

引用

页码：1650 / 1656

页数：7

共 18 条

[1] Standardization of methods for fluence (UV dose) determination in bench-scale UV experiments [J].

Bolton, JR ;

Linden, KG .

JOURNAL OF ENVIRONMENTAL ENGINEERING-ASCE, 2003, 129 (03) :209-215

[2] ENDONUCLEASE FROM MICROCOCCUS-LUTEUS WHICH HAS ACTIVITY TOWARD ULTRAVIOLET-IRRADIATED DEOXYRIBONUCLEIC ACID - PURIFICATION AND PROPERTIES [J].

CARRIER, WL ;

SETLOW, RB .

JOURNAL OF BACTERIOLOGY, 1970, 102 (01) :178-&

[3] In vivo formation and repair of cyclobutane pyrimidine dimers and 6-4 photoproducts measured at the gene and nucleotide level in Escherichia coli [J].

Chandrasekhar, D ;

Van Houten, B .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2000, 450 (1-2) :19-40

[4]

Chang DJ, 2000, JCR-J CLIN RHEUMATOL, V6, P4

[5] QUANTITATION OF RADIATION-INDUCED, CHEMICAL-INDUCED, OR ENZYME-INDUCED SINGLE-STRAND BREAKS IN NONRADIOACTIVE DNA BY ALKALINE GEL-ELECTROPHORESIS - APPLICATION TO PYRIMIDINE DIMERS [J].

FREEMAN, SE ;

BLACKETT, AD ;

MONTELEONE, DC ;

SETLOW, RB ;

SUTHERLAND, BM ;

SUTHERLAND, JC .

ANALYTICAL BIOCHEMISTRY, 1986, 158 (01) :119-129

[6] STATISTICAL STUDY OF SPOT-PLATE TECHNIQUE FOR VIABLE-CELL COUNTS [J].

GAUDY, AF ;

GAUDY, ET ;

ABUNIAAJ, F .

APPLIED MICROBIOLOGY, 1963, 11 (04) :305-&

[7]

Harm W., 1980, BIOL EFFECTS ULTRAVI

[8]

Jagger J., 1967, INTRO RES ULTRAVIOLE

[9] Comparative effectiveness of UV wavelengths for the inactivation of Cryptosporidium parvum oocysts in water [J].

Linden, KG ;

Shin, G ;

Sobsey, MD .

WATER SCIENCE AND TECHNOLOGY, 2001, 43 (12) :171-174

[10] Photoreactivation of Escherichia coli after low- or medium-pressure UV disinfection determined by an endonuclease sensitive site assay [J].

Oguma, K ;

Katayama, H ;

Ohgaki, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (12) :6029-6035

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