Local cyclical compression modulates macrophage function in situ and alleviates immobilization-induced muscle atrophy

被引：18

作者：

Saitou, Kumiko ^{[1
,2
]}

Tokunaga, Masakuni ^{[1
]}

Yoshino, Daisuke ^{[3
]}

Sakitani, Naoyoshi ^{[1
]}

Maekawa, Takahiro ^{[1
]}

Ryu, Youngjae ^{[1
]}

Nagao, Motoshi ^{[1
]}

Nakamoto, Hideki ^{[4
]}

Saito, Taku ^{[4
]}

Kawanishi, Noriaki ^{[5
]}

Suzuki, Katsuhiko ^{[6
]}

Ogata, Toru ^{[1
]}

Makuuchi, Michiru ^{[7
]}

Takashima, Atsushi ^{[8
]}

Sawada, Keisuke ^{[9
]}

Kawamura, Shunsuke ^{[10
]}

Nakazato, Koichi ^{[11
]}

Kouzaki, Karina ^{[11
]}

Harada, Ichiro ^{[12
]}

Ichihara, Yoshinori ^{[1
,13
]}

Sawada, Yasuhiro ^{[1
,14
]}

机构：

[1] Natl Rehabil Ctr Persons Disabil, Dept Rehabil Motor Funct, 4-1 Namiki, Tokorozawa, Saitama 3598555, Japan

[2] Waseda Univ, Grad Sch Sport Sci, 2-579-15 Mikajima, Tokorozawa, Saitama 3591192, Japan

[3] Tohoku Univ, Frontier Res Inst Interdisciplinary Sci, Aoba Ku, 6-3 Aramaki Aza Aoba, Sendai, Miyagi 9808578, Japan

[4] Univ Tokyo, Grad Sch Med, Dept Orthopaed Surg, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan

[5] Chiba Inst Technol, Fac Adv Engn, 2-1-1 Shibazono, Narashino, Chiba 2750023, Japan

[6] Waseda Univ, Fac Sport Sci, 2-579-15 Mikajima, Tokorozawa, Saitama 3591192, Japan

[7] Natl Rehabil Ctr Persons Disabil, Sect Neuropsychol, 4-1 Namiki, Tokorozawa, Saitama 3598555, Japan

[8] Natl Rehabil Ctr Persons Disabil, Dept Assist Technol, 4-1 Namiki, Tokorozawa, Saitama 3598555, Japan

[9] Childrens Hosp Philadelphia, Dept Pathol, Cell Pathol Div, Philaldelphia, PA 19104 USA

[10] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, Mattenstr 26, CH-4058 Basel, Switzerland

[11] Nippon Sport Sci Univ, Grad Sch Hlth & Sport Sci, Setagaya Ku, 7-1-1 Fukasawa, Tokyo 1588508, Japan

[12] Nadogaya Hosp, Nadogaya Inst, 687-4 Nadogaya, Kashiwa, Chiba 2770032, Japan

[13] Tottori Univ, Fac Med, Div Pharmacol, 86 Nishi Cho, Yonago, Tottori 6838503, Japan

[14] Natl Rehabil Ctr Persons Disabil, Dept Clin Res, 4-1 Namiki, Tokorozawa, Saitama 3598555, Japan

来源：

CLINICAL SCIENCE | 2018年 / 132卷 / 19期

基金：

日本学术振兴会;

关键词：

SKELETAL-MUSCLE; INTERSTITIAL FLUID; SHEAR-STRESS; EXERCISE; INFLAMMATION; RECOVERY; MASSAGE; MOUSE; PRESSURE; PROTEIN;

D O I：

10.1042/CS20180432

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Physical inactivity gives rise to numerous diseases and organismal dysfunctions, particularly those related to aging. Musculoskeletal disorders including muscle atrophy, which can result from a sedentary lifestyle, aggravate locomotive malfunction and evoke a vicious circle leading to severe functional disruptions of vital organs such as the brain and cardiovascular system. Although the significance of physical activity is evident, molecular mechanisms behind its beneficial effects are poorly understood. Here, we show that massage-like mechanical interventions modulate immobilization-induced pro-inflammatory responses of macrophages in situ and alleviate muscle atrophy. Local cyclical compression (LCC) on mouse calves, which generates intramuscular pressure waves with amplitude of 50 mmHg, partially restores the myofiber thickness and contracting forces of calf muscles that are decreased by hindlimb immobilization. LCC tempers the increase in the number of cells expressing pro-inflammatory proteins, tumor necrosis factor-alpha and monocyte chemoattractant protein-1 (MCP-1), including macrophages in situ The reversing effect of LCC on immobilization-induced thinning of myofibers is almost completely nullified when macrophages recruited from circulating blood are depleted by administration of clodronate liposomes. Furthermore, application of pulsatile fluid shear stress, but not hydrostatic pressure, reduces the expression of MCP-1 in macrophages in vitro Together with the LCC-induced movement of intramuscular interstitial fluid detected by mu CT analysis, these results suggest that mechanical modulation of macrophage function is involved in physical inactivity-induced muscle atrophy and inflammation. Our findings uncover the implication of mechanosensory function of macrophages in disuse muscle atrophy, thereby opening a new path to develop a novel therapeutic strategy utilizing mechanical interventions.

引用

页码：2147 / 2161

页数：15

共 59 条

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