Cell cycle analysis of Chinese hamster ovary cells stimulated by phosphatidic acid in serum-free culture

被引：3

作者：

Yamaji, H

Sakai, K

Joho, T

Izumoto, E

Fukuda, H

机构：

[1] Kobe Univ, Fac Engn, Dept Sci & Chem Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Grad Sch Sci & Technol, Dept Mol Sci & Mat Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Kaneka Corp, Prod Technol RD Ctr, Takasago, Hyogo 6768688, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2004年 / 98卷 / 06期

关键词：

phosphatidic acid; Chinese hamster ovary (CHO) cells; serum-free culture; cell cycle analysis; flow cytometry;

D O I：

10.1263/jbb.98.487

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

When recombinant Chinese hamster ovary cells were treated with pertussis toxin or genistein, not only lysophosphatidic acid (LPA) but also phosphatidic acid (PA) failed to stimulate progression through the cell cycle in serum-free culture, suggesting that PA and LPA induce cell growth through the same signal transduction pathway. Cell cycle analysis also indicates that cell growth promoted by PA results in enhanced protein production.

引用

页码：487 / 489

页数：3

共 6 条

[1] Lysophosphatidic acid: G-protein signalling and cellular responses [J].