Fe2+ imaging in ferroptosis and drug-induced liver injury with a ratiometric near-infrared fluorescent probe

被引：43

作者：

Feng, Shumin ^{[1
]}

Zheng, Junrong ^{[1
]}

Zhang, Jinzheng ^{[1
]}

Gui, Zhisheng ^{[1
]}

Feng, Guoqiang ^{[1
]}

机构：

[1] Cent China Normal Univ, Coll Chem, Key Lab Pesticide, Chem Biol Minist Educ, 152 Luoyu Rd, Wuhan 430079, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2022年 / 371卷

基金：

中国国家自然科学基金;

关键词：

Ferrous ion; Near-infrared; Ratiometric fluorescence probe; Ferroptosis; Drug-induced liver injury; LABILE IRON(II) POOLS; HIGH-SENSITIVITY; VISUALIZATION; FE(II); CELLS; ION;

D O I：

10.1016/j.snb.2022.132512

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Recently, great attention has been given to the detection of ferrous ion (Fe2+) in live organisms because it plays a vital role in life and is associated with ferroptosis and many diseases. Near-infrared (NIR) ratiometric fluorescence probes are essential tools, however, such probes for tracking Fe2+ in iron-associated diseases in vivo are rarely reported. Herein, we report a novel NIR ratiometric fluorescent probe NRF-Fe2+ for Fe2+ based on a fluorescence resonance energy transfer (FRET) system using the N-oxide chemistry. This probe shows significant ratiometric fluorescence changes (lambda(em) shifts from 460 nm to 675 nm) for Fe2+ with high selectivity and sensitivity, and can image both exogenous and endogenous Fe2+ in cells with low cytotoxicity and ratiometric fluorescence signals. The imaging of Fe2+ in mice with NRF-Fe2+ is also successfully applied. Finally, we demonstrate that the probe can be applied for monitoring the level changes of Fe2+ in ferroptosis and drug-induced liver injury. All the results show that NRF-Fe2+ is a useful tool for the detection of biological Fe2+ and the study of iron-related diseases.

引用

页数：9

共 47 条

[1] Drug-induced liver injury [J].