Effects of liposome-mediated gene transfer of VEGF in ischemic rat gracilis muscle

被引：35
作者：
Neumeister, MW [1 ]
Song, YH [1 ]
Mowlavi, A [1 ]
Suchy, H [1 ]
Mathur, A [1 ]
机构：
[1] So Illinois Univ, Sch Med, Inst Plast & Reconstruct Surg, Springfield, IL 62794 USA
来源：
MICROSURGERY | 2001年 / 21卷 / 02期
关键词：
D O I：
10.1002/micr.1010
中图分类号：
R61 [外科手术学];
学科分类号：
摘要：
The purpose of the current study was to determine the effects of vascular endothelial growth factor (VEGF) on muscle flap survival and vascularity in a rat gracilis ischemia-reperfusion model. A total of 12 adult male Wistar rats were divided into two groups (n = 6). The experimental group received the plasmid encoding VEGF(165) cDNA plus lipofectamine (cationic liposome) injected directly to the gracilis muscle following 4 h of ischemia. The control group received lipofectamine only. The viability and vascularity of the flaps were evaluated after 7 days of reperfusion. The data demonstrated that the VEGF plasmid- and lipofectamine-treated muscle flaps had significantly greater total survival and capillary count 7 days after reperfusion compared with the flaps treated only with lipofectamine. These results indicate that VEGF exerts a protective effect on ischemic skeletal muscle flaps. (C) 2001 Wiley-Liss, Inc.
引用
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页码：58 / 62
页数：5
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