Anti-inflammation Effects of Sinomenine on Macrophages through Suppressing Activated TLR4/NF-κB Signaling Pathway

Sinomenine (SN) has been used in the clinical treatment of systemic lupus erythematosus and rheumatoid arthritis for many years. Studies showed that SN held protective effects such as anti-inflammation, scavenging free radicals and suppressing immune response in many autoimmune diseases. The purpose of the present study is to explore the mechanism of anti-inflammation of SN on lipopolysaccharide (LPS)-induced macrophages activation and investigate whether the TLR4/NF-kappa B signaling pathway participated in. Macrophages isolated from mouse peritoneal cavity were stimulated by 1 mu g/mL LPS for 24 h. And then the cells were treated with various concentrations of SN, TLR4 inhibitor respectively for additional 48 h. Drug toxicity was detected by MTT assay and Transwell experiment was used to assess chemotaxis. Furthermore, TLR4 and MyD88 mRNA levels were detected by real-time PCR. Western blotting was used to examine TLR4, MyD88 and phosphorylated I kappa B protein expression in macrophages. Immunofluorescence assay was applied to observe p65 NF-kappa B protein expression in macrophage nucleus. We extracted macrophages with high purity and activity from the abdominal cavity of mice. SN remarkably inhibited the chemotaxis and secretion function of LPS-stimulated macrophages. It also down-regulated both the protein levels of inflammatory cytokines (TNF-alpha, IL-1 beta and IL-6) and the RNA and protein levels of the key factors (TLR4, MyD88, P-I kappa B) in TLR4 pathway. The expression of p65 NF-kappa B protein in nuclei was down-regulated, which was correlated with a similar decrease in P-I kappa B protein level. In conclusion, SN can inhibit the LPS induced immune responses in macrophages by blocking the activated TLR4/NF-kappa B signaling pathway. These results may provide a therapeutic approach to regulate inflammatory responses.