Large-scale imaging of cortical network activity with calcium indicators

被引：99

作者：

Ikegaya, Y ^{[1
]}

Le Bon-Jego, M ^{[1
]}

Yuste, R ^{[1
]}

机构：

[1] Columbia Univ, Dept Biol Sci, Fairchild Ctr 1002, New York, NY 10027 USA

来源：

NEUROSCIENCE RESEARCH | 2005年 / 52卷 / 02期

关键词：

slice preparation; cortex; fluo-4; Oregon green; spike; microcircuitry; action potential; circuit; spinning disk; confocal microscope;

D O I：

10.1016/j.neures.2005.02.004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

引用

页码：132 / 138

页数：7