An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

被引：71

作者：

Zhang, Liaoyuan ^{[1
,2
]}

Singh, Raushan ^{[2
]}

Sivakumar, D. ^{[2
]}

Guo, Zewang ^{[1
]}

Li, Jiahuan ^{[1
]}

Chen, Fanbing ^{[1
]}

He, Yuanzhi ^{[1
]}

Guan, Xiong ^{[1
]}

Kang, Yun Chan ^{[3
]}

Lee, Jung-Kul ^{[2
]}

机构：

[1] Fujian Agr & Forestry Univ, Gutian Edible Fungi Res Inst, Coll Life Sci, Key Lab Biopesticide & Chem Biol,Minist Educ, Fuzhou 350002, Fujian, Peoples R China

[2] Konkuk Univ, Dept Chem Engn, Seoul 05029, South Korea

[3] Korea Univ, Dept Mat Sci & Engn, Seoul 02841, South Korea

来源：

GREEN CHEMISTRY | 2018年 / 20卷 / 01期

基金：

新加坡国家研究基金会; 中国国家自然科学基金;

关键词：

HIGH-YIELD PRODUCTION; ESCHERICHIA-COLI; ALCOHOL-DEHYDROGENASE; ENZYME IMMOBILIZATION; KLEBSIELLA-PNEUMONIAE; GLYCEROL; BIOFUELS; DEHYDRATASE; RESOURCES; PARTICLES;

D O I：

10.1039/c7gc02898a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

引用

页码：230 / 242

页数：13

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