Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins

被引:57
作者
Abuna, Gabriel [1 ]
Feitosa, Victor P. [2 ]
Correr, Americo Bortolazzo [1 ]
Cama, Giuseppe [3 ]
Giannini, Marcelo [1 ]
Sinhoreti, Mario A. [1 ]
Pashley, David H. [4 ]
Sauro, Salvatore [5 ]
机构
[1] Campinas State Univ UNICAMP, Sch Dent, Dent Mat Div, Dept Restorat Dent, Piracicaba, SP, Brazil
[2] Univ Fed Ceara, Dept Restorat Dent, Fortaleza, Ceara, Brazil
[3] Univ Ghent, Polymer Chem & Biomat Grp, Ghent, Belgium
[4] Dent Coll Georgia, Dept Oral Biol, Augusta, GA USA
[5] Univ CEU Cardenal Herrera, Dept Odontol, Fac Ciencias Salud, Dent Biomat Prevent & Minimally Invas Dent, Valencia, Spain
关键词
Adhesive; Biomimetic remineralization; Collagen; Dentin; Self-etching; DENTIN; REMINERALIZATION; COLLAGEN; SYSTEMS; MINERALIZATION; DEGRADATION; MECHANISM; MATRICES; TISSUE;
D O I
10.1016/j.jdent.2016.07.016
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives: This study examined the bonding performance and dentin remineralization potential of an experimental adhesive containing calcium-phosphate (Ca/P) micro-fillers, and self-etching primers doped with phosphoprotein biomimetic analogs (polyacrylic acid-(PAA) and/or sodium trimetaphosphate-(TMP)). Methods: Experimental self-etching primers doped with biomimetic analogs (PAA and/or TMP), and an adhesive containing Ca2+, PO4-3-releasing micro-fillers (Ca/P) were formulated. Sound human dentin specimens were bonded and cut into sticks after aging (24 h or 6 months) under simulated pulpal pressure (20 cm H2O), and tested for microtensile bond strength (mu TBS). Results were analyzed using two-way ANOVA and Tukey's test (p < 0.05). Interfacial silver nanoleakage was assessed using SEM. Remineralization of EDTA-demineralized dentin was assessed through FTIR and TEM ultrastructural analysis. Results: Application of the Ca/P-doped adhesive with or without dentin pre-treatments with the primer containing both biomimetic analogs (PAA and TMP) promoted stable mTBS over 6 months. Conversely, mTBS of the control primer and filler-free adhesive significantly decreased after 6 months. Nanoleakage decreased within the resin-dentin interfaces created using the Ca/P-doped adhesives. EDTA-demineralized dentin specimens treated the Ca/P-doped adhesive and the primer containing PAA and TMP showed phosphate uptake (FTIR analysis), as well as deposition of needle-like crystallites at intrafibrillar level (TEM analysis). Significance: The use of Ca/P-doped self-etching adhesives applied in combination with analogs of phosphoproteins provides durable resin-dentin bonds. This approach may represent a suitable bonding strategy for remineralization of intrafibrillar dentin collagen within the resin-dentin interface. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:79 / 86
页数:8
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