Expression and characterization of inhA gene from Bacillus thuringiensis 8010

被引：7
作者：
Yu, Xiaomin
Huang, Tianpei
Huang, Zhipeng
Powell, Charles A.
Guan, Xiong [1 ]
机构：
[1] Minist Educ, Fujian Agr & Forestry Univ, Key Lab Bbiopesticide & Chem Biol, Fujian 350002, Peoples R China
[2] Univ Florida, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2007年 / 23卷 / 11期
基金：
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词：
Bacillus thuringiensis; bioassay; expression; inhA gene; sequence analysis;
D O I：
10.1007/s11274-007-9408-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
InhA, a zinc metalloprotease secreted by Bacillus thuringiensis, specifically hydrolyzes antibacterial peptides produced by insect hosts. In this study, the inhA gene was cloned from B. thuringiensis 8010 using a pair of degenerate primers and the deduced 796 amino acid sequence showed a high degree of similarity with other InhA proteins in the Bacillus cereus group. The deduced amino acid sequence contained the zinc-binding motif (HEXXH), which is characteristic of the zinc-metalloprotease family. Additionally, the inhA gene was expressed in Escherichia coli BL21 (DE3). The expressed InhA protein was shown to be toxic to the third larvae of Plutella xylostella, contrary to preliminary study concerning the effect of InhA on Bombyx mori. This study provided insights into the potential of InhA for the biological control of certain lepidopteran insects.
引用
收藏
页码：1621 / 1625
页数：5
相关论文
共 17 条
[1]   PlcR is a pleiotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis [J].
Agaisse, H ;
Gominet, M ;
Okstad, OA ;
Kolsto, AB ;
Lereclus, D .
MOLECULAR MICROBIOLOGY, 1999, 32 (05) :1043-1053
[2]   CHARACTERIZATION OF INHIBITOR-A, A PROTEASE FROM BACILLUS-THURINGIENSIS WHICH DEGRADES ATTACINS AND CECROPINS, 2 CLASSES OF ANTIBACTERIAL PROTEINS IN INSECTS [J].
DALHAMMAR, G ;
STEINER, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 139 (02) :247-252
[3]   EVIDENCE FOR 2 IMMUNE INHIBITORS FROM BACILLUS-THURINGIENSIS INTERFERING WITH HUMORAL DEFENSE SYSTEM OF SATURNIID PUPAE [J].
EDLUND, T ;
SIDEN, I ;
BOMAN, HG .
INFECTION AND IMMUNITY, 1976, 14 (04) :934-941
[4]   The Bacillus thuringiensis PlcR-regulated gene inhA2 is necessary, but not sufficient, for virulence [J].
Fedhila, S ;
Gohar, M ;
Slamti, L ;
Nel, P ;
Lereclus, D .
JOURNAL OF BACTERIOLOGY, 2003, 185 (09) :2820-2825
[5]   The InhA2 metalloprotease of Bacillus thuringiensis strain 407 is required for pathogenicity in insects infected via the oral route [J].
Fedhila, S ;
Nel, P ;
Lereclus, D .
JOURNAL OF BACTERIOLOGY, 2002, 184 (12) :3296-3304
[6]   Identification of genes involved in the activation of the Bacillus thuringiensis inhA metalloprotease gene at the onset of sporulation [J].
Grandvalet, C ;
Gominet, M ;
Lereclus, D .
MICROBIOLOGY-SGM, 2001, 147 :1805-1813
[7]  
Guan X., 1997, STUDIES BACILLUS THU
[8]   A UNIQUE SIGNATURE IDENTIFIES A FAMILY OF ZINC-DEPENDENT METALLOPEPTIDASES [J].
JONGENEEL, CV ;
BOUVIER, J ;
BAIROCH, A .
FEBS LETTERS, 1989, 242 (02) :211-214
[9]   ENHANCED PRODUCTION OF INSECTICIDAL PROTEINS IN BACILLUS-THURINGIENSIS STRAINS CARRYING AN ADDITIONAL CRYSTAL PROTEIN GENE IN THEIR CHROMOSOMES [J].
KALMAN, S ;
KIEHNE, KL ;
COOPER, N ;
REYNOSO, MS ;
YAMAMOTO, T .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (08) :3063-3068
[10]   MOLECULAR CHARACTERIZATION OF IMMUNE INHIBITOR-A, A SECRETED VIRULENCE PROTEASE FROM BACILLUS-THURINGIENSIS [J].
LOVGREN, A ;
ZHANG, M ;
ENGSTROM, A ;
DALHAMMAR, G ;
LANDEN, R .
MOLECULAR MICROBIOLOGY, 1990, 4 (12) :2137-2146
12