Methyl jasmonate and miconazole differently affect arteminisin production and gene expression in Artemisia annua suspension cultures

被引：64

作者：

Caretto, S. ^{[1
]}

Quarta, A. ^{[1
]}

Durante, M. ^{[1
]}

Nisi, R. ^{[1
]}

De Paolis, A. ^{[1
]}

Blando, F. ^{[1
]}

Mita, G. ^{[1
]}

机构：

[1] CNR, Ist Sci Prod Alimentari, I-73100 Lecce, Italy

来源：

PLANT BIOLOGY | 2011年 / 13卷 / 01期

关键词：

Gene expression; malaria; plant cell cultures; HAIRY ROOT CULTURES; AMORPHA-4,11-DIENE SYNTHASE; MOLECULAR-CLONING; BIOSYNTHESIS; PLANT; ACID; GROWTH; CDNAS;

D O I：

10.1111/j.1438-8677.2009.00306.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Artemisia annua L. is a herb traditionally used for treatment of fevers. The glandular trichomes of this plant accumulate, although at low levels, artemisinin, which is highly effective against malaria. Due to the great importance of this compound, many efforts have been made to improve knowledge on artemisinin production both in plants and in cell cultures. In this study, A. annua suspension cultures were established in order to investigate the effects of methyl jasmonate (MeJA) and miconazole on artemisinin biosynthesis. Twenty-two micro molar MeJA induced a three-fold increase of artemisinin production in around 30 min; while 200 mu m miconazole induced a 2.5-fold increase of artemisinin production after 24 h, but had severe effects on cell viability. The influence of these treatments on expression of biosynthetic genes was also investigated. MeJA induced up-regulation of CYP71AV1, while miconazole induced up-regulation of CPR and DBR2.

引用

页码：51 / 58

页数：8

共 39 条

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