P2X(1) receptor activation in HL60 cells

被引：76

作者：

Buell, G ^{[1
]}

Michel, AD ^{[1
]}

Lewis, C ^{[1
]}

Collo, G ^{[1
]}

Humphrey, PPA ^{[1
]}

Surprenant, A ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT PHARMACOL,GLAXO INST APPL PHARMACOL,CAMBRIDGE,ENGLAND

来源：

BLOOD | 1996年 / 87卷 / 07期

关键词：

D O I：

10.1182/blood.V87.7.2659.bloodjournal8772659

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Recent cloning of the human P2X(1) receptor revealed high levels of its messenger RNA in differentiated promyelocytes (HL60 cells), We found expression of P2X(1) receptor protein in HL60 cells by radioligand binding, by immunohistochemistry, using a receptor specific antibody, and by electrophysiology, The currents elicited by adenosine triphosphate (ATP) had the expected properties of P2X(1) receptors (rapid desensitization, mimicked by alpha,beta-methylene-ATP). However, these currents were only observed in cells that were pretreated with apyrase, which destroys extracellular ATP, or with suramin, a P2X receptor antagonist, This implies that HL60 cells release ATP, which chronically desensitizes the receptor, ATP release was detected by direct measurement, using the luciferin-luciferase assay. It is concluded that functional P2X(1) receptors are present in the membrane of differentiated HL60 cells. (C) 1996 by The American Society of Hematology.

引用

页码：2659 / 2664

页数：6

共 27 条

[1]

ALONSOTORRE SR, 1993, J BIOL CHEM, V268, P18640

[2] ROLE OF EXTRACELLULAR ATP AND P1 AND P2 CLASSES OF PURINERGIC RECEPTORS IN T-CELL DEVELOPMENT AND CYTOTOXIC T-LYMPHOCYTE EFFECTOR FUNCTIONS [J].

APASOV, S ;

KOSHIBA, M ;

REDEGELD, F ;

SITKOVSKY, MV .

IMMUNOLOGICAL REVIEWS, 1995, 146 :5-19

[3]

BARICORDI R, 1996, IN PRESS BLOOD

[4]

BENNET J, 1995, J PHYSL, V317, P335

[5] NEW STRUCTURAL MOTIF FOR LIGAND-GATED ION CHANNELS DEFINED BY AN IONOTROPIC ATP RECEPTOR [J].

BRAKE, AJ ;

WAGENBACH, MJ ;

JULIUS, D .

NATURE, 1994, 371 (6497) :519-523

[6] NONSELECTIVE CATIONIC CURRENTS ELICITED BY EXTRACELLULAR ATP IN HUMAN AND B-LYMPHOCYTES [J].

BRETSCHNEIDER, F ;

KLAPPERSTUCK, M ;

LOHN, M ;

MARKWARDT, F .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1995, 429 (05) :691-698

[7]

BURGERS JA, 1993, BLOOD, V81, P49

[8] A P2X PURINOCEPTOR EXPRESSED BY A SUBSET OF SENSORY NEURONS [J].

CHEN, CC ;

AKOPIAN, AN ;

SIVILOTTI, L ;

COLQUHOUN, D ;

BURNSTOCK, G ;

WOOD, JN .

NATURE, 1995, 377 (6548) :428-431

[9] ATP STIMULATES SECRETION IN HUMAN-NEUTROPHILS AND HL60 CELLS VIA A PERTUSSIS TOXIN-SENSITIVE GUANINE NUCLEOTIDE-BINDING PROTEIN COUPLED TO PHOSPHOLIPASE-C [J].

COCKCROFT, S ;

STUTCHFIELD, J .

FEBS LETTERS, 1989, 245 (1-2) :25-29

[10] THE P2Z PURINOCEPTOR - AN INTRIGUING ROLE IN IMMUNITY, INFLAMMATION AND CELL-DEATH [J].

DIVIRGILIO, F .

IMMUNOLOGY TODAY, 1995, 16 (11) :524-528

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