HTRF®: pioneering technology for high-throughput screening

被引:25
|
作者
Degorce, Francois [1 ]
机构
[1] Vitro Technol, Cisbio Int, BP 84175, F-30240 Bagnols Sur Ceze, France
关键词
assay; fluorescence; FRET; GPCR screening; HTS; kinase screening; TR-FRET;
D O I
10.1517/17460441.1.7.753
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Cisbio international pioneered the field of homogeneous fluorescence methodologies and time-resolved fluorescence resonance in particular, through its proprietary technology, HTRF (R). The development was based on Professor Jean-Marie Lehn's research on rare earth fluorescence properties (awarded the Nobel Prize in Chemistry in 1987) and on Cisbio's expertise in homogenous time-resolved fluorescence (HTRF). The technology is used in assay development and drug screening, most notably in high-throughput screening applications. This highly powerful technology is particularly applied to the areas of G-protein-coupled receptor and kinase screening, as well as a series of targets related to inflammation, metabolic diseases and CNS disorders.
引用
收藏
页码:753 / 764
页数:12
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