A label-free biosensor based on gold nanoshell monolayers for monitoring biomolecular interactions in diluted whole blood

被引：91
作者：
Wang, Yi [1 ,2 ]
Qian, Weiping [1 ]
Tan, Yong [1 ]
Ding, Shaohua [1 ]
机构：
[1] SE Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Peoples R China
[2] SE Univ, Sch Chem & Chem Engn, Nanjing 210096, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 07期
基金：
中国国家自然科学基金;
关键词：
gold nanoshells; self-assembled monolayer; biosensor; localized surface plasmon resonance; streptavidin; biotin;
D O I：
10.1016/j.bios.2007.10.020
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Gold nanoshells (GNSs) were self-assembled on the surface of transparent glasses modified with 3-aminopropyltrimethoxysitane (APTES) to form GNS self-assembled monolayers (SAMs). Because the localized surface plasmon resonance (LSPR) of GNSs can be controlled in the near-infrared (NIR) region of the spectrum, where the optical transmission through tissue and whole blood is optimal, GNSs would be used as an effective signal transduction in whole blood. Accordingly, after modified with cystamine and biotin-NHS (N-hydroxy succinimide), GNS SAMs were used as a novel optical biosensor for real-time detection of streptavidin-biotin interactions in diluted human whole blood within short assay time, without any sample purification/separation. An UV-vis-NIR spectrophotometer was used to monitor the absorbance changes at 730 nm as a function of time for different concentrations of streptavidin in 20% whole blood, and the results showed that the biosensor displayed low detection limit of similar to 3 mu g/mL and wide dynamic range of similar to 3-50 mu g/mL. This approach provides an opportunity to construct LSPR biosensor for protein sensing and cellular analysis in diluted whole blood. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：1166 / 1170
页数：5
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